Yang Z F, Schügerl K, Lucas L
Institut für Technische Chemie, Universität Hannover, Germany.
J Biotechnol. 1996 Nov 1;51(2):137-48. doi: 10.1016/0168-1656(96)01592-1.
The inhibitory mechanism of glucose and CO2 on the biosyntheses of penicillins and cephalosporins is discussed in the present paper. 6-aminopenicillanic acid (6-APA) is considered to be an intermediate product, and the reaction between 6-APA and glucose may play an important role in the yield and rate of biosyntheses of beta-lactam antibiotics. According to this hypothesis the experimental phenomena taking place in biosynthesis of penicillin and cephalosporin, such as the inhibition by glucose and carbon dioxide and the reduction of the yield, can be satisfactorily explained. The stability of 6-aminopenicillanic acid (6-APA) in bicarbonate solution, the reaction of 6-APA with sugars, the determination of the concentration of the 6-APA-sugar compound and the effect of these reactions on the biosynthesis of penicillin G are investigated to present evidences for this hypothesis.
本文讨论了葡萄糖和二氧化碳对青霉素和头孢菌素生物合成的抑制机制。6-氨基青霉烷酸（6-APA）被认为是一种中间产物，6-APA与葡萄糖之间的反应可能在β-内酰胺抗生素生物合成的产量和速率中起重要作用。根据这一假设，可以令人满意地解释在青霉素和头孢菌素生物合成中发生的实验现象，如葡萄糖和二氧化碳的抑制作用以及产量的降低。研究了6-氨基青霉烷酸（6-APA）在碳酸氢盐溶液中的稳定性、6-APA与糖的反应、6-APA-糖化合物浓度的测定以及这些反应对青霉素G生物合成的影响，以提供支持这一假设的证据。
