Wang F L, Dombrowski M P, Hurd W W
Department of Obstetrics and Gynecology, University of Michigan Medical Center, Ann Arbor, Michigan, USA.
Am J Obstet Gynecol. 1996 Dec;175(6):1651-3. doi: 10.1016/s0002-9378(96)70120-5.
Our purpose was to evaluate the in vitro effect of cocaine on beta-adrenergic receptor function in pregnant human myometrium.
Myometrium was obtained from 25 pregnant women at term who were undergoing cesarean section but who were not in labor. Membrane preparations were assayed for cyclic adenosine monophosphate production after exposure to isoproterenol, sodium fluoride, or forskolin in the presence and absence of either cocaine or benzoylecgonine, a major metabolite of cocaine.
Cocaine inhibited isoproterenol-induced cyclic adenosine monophosphate production in concentrations as low as 10(-8) mol/L but had no effect on cyclic adenosine monophosphate production induced by sodium fluoride or forskolin. Benzoylecgonine (10(-4) mol/L) had no effect on isoproterenol-induced cyclic adenosine monophosphate production.
Cocaine is a beta-adrenergic receptor antagonist in human myometrium at concentrations as low as 10(-8) mol/L. This acute effect of cocaine may result in increased uterine contractility with adrenergic stimulation, potentially leading to increased uterine activity associated with acute cocaine use.
我们的目的是评估可卡因对妊娠人类子宫肌层β-肾上腺素能受体功能的体外作用。
从25名足月行剖宫产但未临产的孕妇获取子宫肌层。在存在和不存在可卡因或苯甲酰爱康宁（可卡因的主要代谢产物）的情况下，将膜制剂暴露于异丙肾上腺素、氟化钠或福斯高林后，检测环磷酸腺苷的产生。
可卡因在低至10^(-8)摩尔/升的浓度下即可抑制异丙肾上腺素诱导的环磷酸腺苷产生，但对氟化钠或福斯高林诱导的环磷酸腺苷产生无影响。苯甲酰爱康宁（10^(-4)摩尔/升）对异丙肾上腺素诱导的环磷酸腺苷产生无影响。
可卡因在低至10^(-8)摩尔/升的浓度下是人类子宫肌层中的β-肾上腺素能受体拮抗剂。可卡因的这种急性作用可能导致肾上腺素能刺激时子宫收缩性增加，潜在地导致与急性可卡因使用相关的子宫活动增加。
