来自人脑的II型环磷酸腺苷依赖性蛋白激酶调节亚基的特性

Properties of the regulatory subunit of cAMP-dependent protein kinase type II from human brain.

作者信息

Grozdova I D, Alexandrova N A, Sveshnikova E V, Melik-Nubarov N S, Sveshnikov P G, Severin E S

机构信息

Russian Research Center of Molecular Diagnostics and Therapy, Moscow, Russia.

出版信息

Biochem Mol Biol Int. 1996 Dec;40(6):1159-66. doi: 10.1080/15216549600201793.

DOI:10.1080/15216549600201793

PMID:8988327

Abstract

The regulatory subunit type II (RII) of cAMP-dependent protein kinase purified from human brain was represented by two proteins with apparent molecular masses of 51-52 kD and 54 kD. Dephosphorylation of human RII containing 3 mol phosphate/mol protein did not change the electrophoretic pattern. One-dimensional peptide mapping of 51-52 kD and 54 kD proteins after digestion with St. aureus V8 protease evidenced to their being distinct proteins. The data obtained permit to assume that human RII of neural type is represented by two isoforms.

摘要

从人脑中纯化得到的环磷酸腺苷（cAMP）依赖性蛋白激酶的调节亚基II型（RII）由两种蛋白质组成，其表观分子量分别为51 - 52 kD和54 kD。含3摩尔磷酸/摩尔蛋白质的人RII去磷酸化后，电泳图谱未发生改变。用金黄色葡萄球菌V8蛋白酶消化后，对51 - 52 kD和54 kD蛋白质进行一维肽图分析，证明它们是不同的蛋白质。所得数据表明，神经型人RII由两种同工型组成。