Zenmyo M, Hiraoka K, Sasaguri T, Komiya S, Inoue A, Morimatsu M, Sasaguri Y
Department of Pathology, School of Medicine, Kurume University, Japan.
Biochem Mol Biol Int. 1996 Dec;40(6):1253-60. doi: 10.1080/15216549600201903.
In the present study, we investigated the effect of interleukin-4 (IL-4) on metalloproteinase 1 (MMP-1/tissue collagenase) production in human rheumatoid synovial fibroblasts. Northern blot analysis revealed that addition of IL-4 with or without histamine stimulated the cells to increase the amount of proMMP-1 mRNA, and the IL-4 with histamine addition resulted in a 3.3-fold increase compared with histamine only. Furthermore, IL-4 itself stimulated the expression of histamine H1 receptor mRNA. These results suggest that IL-4 may play an important role in joint destruction in RA.
在本研究中，我们调查了白细胞介素-4（IL-4）对人类风湿性滑膜成纤维细胞中金属蛋白酶1（MMP-1/组织胶原酶）产生的影响。Northern印迹分析显示，添加有或没有组胺的IL-4刺激细胞增加前MMP-1 mRNA的量，并且添加组胺的IL-4导致与仅组胺相比增加了3.3倍。此外，IL-4本身刺激组胺H1受体mRNA的表达。这些结果表明，IL-4可能在类风湿性关节炎的关节破坏中起重要作用。
