针对粒细胞抗原系统的细胞毒素：采用细胞松弛素B处理细胞的新方法进行检测

Cytotoxins against a granulocyte antigen system: detection by a new method employing cytochalasin-B-treated cells.

作者信息

Caplan S N, Berkman E M, Babior B M

出版信息

Vox Sang. 1977 Oct;33(4):206-11. doi: 10.1111/j.1423-0410.1977.tb04465.x.

DOI:10.1111/j.1423-0410.1977.tb04465.x

Abstract

A method for demonstrating granulocyte cytotoxins using a modified microdroplet dye exclusion technique and cytochalasin-B-treated cells is described. Cytochalasin-B-treated granulocytes were reacted against 223 sera from multitransfused pregnant and renal transplant patients. Incidence of granulocyte cytotoxins was 11.2%. Analysis (2 X 2 contingency tables) of the reactivity of GCT+ sera showed highly significant (p less than 0.001) positive associations among 5 sera and a significant (p less than 0.001) negative association with another serum. The data are consistent with the hypothesis that a granulocyte antigen system is detected by cytotoxins and that granulocyte antigens 'Grl' and 'Gr2' may be products of allelic genes.

摘要

本文描述了一种使用改良的微滴染料排除技术和细胞松弛素B处理的细胞来展示粒细胞毒素的方法。用细胞松弛素B处理的粒细胞与来自多次输血的孕妇和肾移植患者的223份血清进行反应。粒细胞毒素的发生率为11.2%。对GCT+血清反应性的分析（2×2列联表）显示，5份血清之间存在高度显著（p<0.001）的正相关，与另一份血清存在显著（p<0.001）的负相关。这些数据与以下假设一致：细胞毒素检测到一种粒细胞抗原系统，并且粒细胞抗原“Grl”和“Gr2”可能是等位基因的产物。