Saika S, Ooshima A, Shima K, Tanaka S, Ohnishi Y
Department of Ophthalmology, Wakayama Medical College, Japan.
Jpn J Ophthalmol. 1996;40(3):303-9.
We evaluated the change in the type of collagen found during healing in the alkali-burned corneal stroma of rabbits. We estimated the relative proportions of alpha chains in pepsin-solubilized collagen, using sodium dodecylsulfate polyacrylamide gel electrophoresis. Bands of alpha 1(I), alpha 2(I), alpha 1(V), and alpha 1(III) chains stained with Coomassie Blue were separated. The alpha 1(III) and alpha 1(V) chains showed a transient proportional increase in healing corneal stroma in the area exposed to alkali. No significant alterations in collagen alpha chains were detected in peripheral corneal stroma that had not been directly exposed to alkali. Our results suggest that the keratocytes which repopulate the alkali-injured corneal stroma early in healing synthesize a higher proportion of collagen types III and V than type I, and then switch to synthesizing predominantly type I collagen as stromal healing progresses. Collagen types III and V thus appear to be of primary importance in the healing of the corneal stroma.
我们评估了在兔碱烧伤角膜基质愈合过程中发现的胶原蛋白类型的变化。我们使用十二烷基硫酸钠聚丙烯酰胺凝胶电泳法估计了胃蛋白酶可溶解的胶原蛋白中α链的相对比例。用考马斯亮蓝染色的α1（I）、α2（I）、α1（V）和α1（III）链的条带被分离出来。在暴露于碱的区域，愈合的角膜基质中α1（III）和α1（V）链呈现出短暂的比例增加。在未直接暴露于碱的周边角膜基质中，未检测到胶原蛋白α链有明显变化。我们的结果表明，在愈合早期重新填充碱损伤角膜基质的角膜细胞合成的III型和V型胶原蛋白比例高于I型，然后随着基质愈合的进展，转而主要合成I型胶原蛋白。因此，III型和V型胶原蛋白在角膜基质愈合中似乎至关重要。
