Naito J, Ishikawa H, Ohmori Y, Watanabe T
Laboratory of Animal Morphology and Function, School of Agricultural Sciences, Nagoya University, Japan.
J Auton Nerv Syst. 1996 Dec 14;61(3):321-3. doi: 10.1016/s0165-1838(96)00077-x.
Hypothalamic neurons projecting to the lumbar (lumbar-neurons) and sacral (sacral-neurons) segments were retrogradely labeled by injections of fluorescent axonal tracers (FITC-WGA, TRITC-WGA) into both the lumbar and sacral segments. Labeled neurons were distributed in the principal part of the paraventricular nucleus (PVN), but not in the pars dispersa of this nucleus. Of these neurons, some small and oval-shaped neurons (about 10 microns in diameter) in a middle part of the PVN were observed to be labeled by both FITC-WGA and TRITC-WGA (lumbosacral-neurons) suggesting double projections to lumbar and sacral segments.
通过将荧光轴突示踪剂(FITC-WGA、TRITC-WGA)注射到腰段和骶段,对投射至腰段(腰神经元)和骶段(骶神经元)的下丘脑神经元进行逆行标记。标记的神经元分布于室旁核(PVN)的主体部分,而非该核的分散部。在这些神经元中,观察到PVN中部一些小的椭圆形神经元(直径约10微米)同时被FITC-WGA和TRITC-WGA标记(腰骶神经元),提示其向腰段和骶段有双重投射。