Wagner J L, Burnett R C, Works J D, Storb R
Transplantation Biology Program, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
Tissue Antigens. 1996 Nov;48(5):554-61. doi: 10.1111/j.1399-0039.1996.tb02669.x.
The polymorphism of the canine major histocompatibility complex class II DRB gene, DRBB1, was analyzed. Exon 2 that encodes the polymorphic beta 1 domain was amplified by the polymerase chain reaction (PCR). The PCR product from 250 dogs was cloned and sequenced. Eighteen alleles were identified. Most of the variation in amino acid composition occurred at positions in the peptide binding site. Inheritance of these sequences showed Mendelian segregation with one or two alleles per dog. Cluster analysis of the nucleotide and predicted amino acid sequences subdivided the canine DRBB1 alleles into three major allelic groups. The number of nonsynonymous changes was higher than the number of synonymous changes in the putative antigen recognition sites indicative of positive selection. The data generated can serve as a basis for developing a typing assay for the canine DRBB1 gene.
对犬主要组织相容性复合体II类DRB基因DRBB1的多态性进行了分析。通过聚合酶链反应（PCR）扩增编码多态性β1结构域的外显子2。对250只犬的PCR产物进行克隆和测序。鉴定出18个等位基因。氨基酸组成的大多数变异发生在肽结合位点的位置。这些序列的遗传显示出孟德尔分离，每只犬有一个或两个等位基因。对核苷酸和预测的氨基酸序列进行聚类分析，将犬DRBB1等位基因细分为三个主要等位基因组。在推定的抗原识别位点，非同义变化的数量高于同义变化的数量，表明存在正选择。所产生的数据可为开发犬DRBB1基因分型检测方法提供依据。
