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墨西哥皱果苋种子中一种凝集素的纯化与特性分析

Purification and characterization of a lectin from Amaranthus hypochondriacus var. Mexico seeds.

作者信息

Ozeki M, Kamemura K, Moriyama K, Itoh Y, Furuichi Y, Umekawa H, Takahashi T

机构信息

Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Japan.

出版信息

Biosci Biotechnol Biochem. 1996 Dec;60(12):2048-51. doi: 10.1271/bbb.60.2048.

DOI:10.1271/bbb.60.2048
PMID:8988637
Abstract

A lectin from Amaranthus hypochondriacus var. Mexico (AHML) was purified by affinity chromatography using asialofetuin-Sepharose 4B. AHML is specific for N-acetyl-D-galactosamine as are the other Amaranthus lectins. AHML has no carbohydrate moiety and requires no metal ion for the hemagglutination activity. The pI of AHML is 6.8. AHML has a native molecular mass of 45.0 kDa and is composed of homo-subunits having molecular masses of 36.8 kDa.

摘要

利用去唾液酸胎球蛋白-琼脂糖凝胶4B通过亲和层析法纯化了来自墨西哥皱果苋变种(AHML)的一种凝集素。AHML与其他苋属凝集素一样,对N-乙酰-D-半乳糖胺具有特异性。AHML没有碳水化合物部分,并且其血凝活性不需要金属离子。AHML的等电点为6.8。AHML的天然分子量为45.0 kDa,由分子量为36.8 kDa的同型亚基组成。

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