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从烟草中细菌生产及纯化可磷酸化的磷酸烯醇式丙酮酸羧化酶

Bacterial production and purification of phosphorylatable phosphoenolpyruvate carboxylase from tobacco.

作者信息

Koizumi N, Sato F, Yamada Y

机构信息

Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University, Japan.

出版信息

Biosci Biotechnol Biochem. 1996 Dec;60(12):2089-91. doi: 10.1271/bbb.60.2089.

DOI:10.1271/bbb.60.2089
PMID:8988646
Abstract

Tobacco phosphoenolpyruvate carboxylase (PEPC) [EC 4.1.1.31] cDNA was efficiently expressed in E. coli under the control of the lacZ promoter. The enzyme, purified to homogeneity, had the same catalytic activity, and was phosphorylated in vitro by maize PEPC kinase.

摘要

烟草磷酸烯醇式丙酮酸羧化酶（PEPC）[EC 4.1.1.31]的cDNA在乳糖操纵子启动子的控制下于大肠杆菌中高效表达。该酶纯化至同质，具有相同的催化活性，并在体外被玉米PEPC激酶磷酸化。

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Bacterial production and purification of phosphorylatable phosphoenolpyruvate carboxylase from tobacco.从烟草中细菌生产及纯化可磷酸化的磷酸烯醇式丙酮酸羧化酶
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引用本文的文献

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Purification and characterization of high- and low-molecular-mass isoforms of phosphoenolpyruvate carboxylase from Chlamydomonas reinhardtii. Kinetic, structural and immunological evidence that the green algal enzyme is distinct from the prokaryotic and higher plant enzymes.莱茵衣藻磷酸烯醇式丙酮酸羧化酶高分子量和低分子量亚型的纯化与表征。动力学、结构和免疫学证据表明，这种绿藻酶与原核生物和高等植物的酶不同。
Biochem J. 1998 Apr 1;331 ( Pt 1)(Pt 1):201-9. doi: 10.1042/bj3310201.