Rovid A H, Roth J A
Department of Microbiology, Immunology, and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, USA.
J Acquir Immune Defic Syndr Hum Retrovirol. 1997 Jan 1;14(1):18-25. doi: 10.1097/00042560-199701010-00004.
Supernatants from bovine immunodeficiency-like virus (BIV)-infected cells have been previously shown to affect monocyte random migration, chemotaxis, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC) in vitro. The experiments in this report demonstrate that the BIV Gag (core) proteins can enhance monocyte random migration, chemotaxis, and phagocytosis. Supernatants from BIV-infected cells contained 10-30 and 30-50 kDa proteins, which significantly (p < 0.05) increased monocyte chemotaxis. The 30-50 kDa protein(s) could be cleaved by limited proteolysis into 10-30 kDa active components. Affinity purification with monoclonal anti-p26 (capsid) antibodies yielded preparations that were active in the random migration, chemotaxis, and phagocytosis assays, but did not affect ADCC. Furthermore, activity of the affinity purified preparation could be specifically neutralized by hyperimmune rabbit serum against BIV Gag proteins. A recombinant Gag protein, consisting primarily of BIV p26, also enhanced monocyte random and chemotactic migration. It appears, therefore, that direct treatment with affinity-purified BIV Gag proteins or a recombinant Gag protein, is able to significantly affect the function of normal monocytes in vitro. Factors affecting monocyte migration and phagocytosis appear to be one or more breakdown products of the BIV Gag precursor, particularly those containing the p26 (capsid) protein.
先前已表明，来自牛免疫缺陷样病毒（BIV）感染细胞的上清液在体外会影响单核细胞的随机迁移、趋化性、吞噬作用以及抗体依赖性细胞介导的细胞毒性（ADCC）。本报告中的实验表明，BIV Gag（核心）蛋白可增强单核细胞的随机迁移、趋化性和吞噬作用。BIV感染细胞的上清液含有10 - 30 kDa和30 - 50 kDa的蛋白质，这些蛋白质能显著（p < 0.05）增加单核细胞的趋化性。30 - 50 kDa的蛋白质可通过有限的蛋白水解作用裂解为10 - 30 kDa的活性成分。用单克隆抗p26（衣壳）抗体进行亲和纯化后得到的制剂，在随机迁移、趋化性和吞噬作用测定中具有活性，但不影响ADCC。此外，亲和纯化制剂的活性可被抗BIV Gag蛋白的超免疫兔血清特异性中和。一种主要由BIV p26组成的重组Gag蛋白，也增强了单核细胞的随机迁移和趋化迁移。因此，似乎用亲和纯化的BIV Gag蛋白或重组Gag蛋白直接处理，能够在体外显著影响正常单核细胞的功能。影响单核细胞迁移和吞噬作用的因素似乎是BIV Gag前体的一种或多种降解产物，特别是那些含有p26（衣壳）蛋白的产物。
