Zwerner R K, Hudson J C, Coulter W H, Brunhouse R F, Russell T R
Coulter Corporation, Miami, Florida, USA.
J Acquir Immune Defic Syndr Hum Retrovirol. 1997 Jan 1;14(1):31-4. doi: 10.1097/00042560-199701010-00006.
Flow cytometry, the method of choice for determining subset cell populations, requires sophisticated instrumentation and highly trained operators. An alternative method for subpopulation determinations is presented that is fast, simple, and readily interpreted for absolute subset counts. This method uses antibody labeled microspheres added to whole blood and after lysing, read on a hemocytometer using an ordinary light microscope. The total time of testing is less than 10 min with results comparable to that obtained using a flow cytometer and a hematology analyzer to calculate absolute subpopulation values. This method is demonstrated for CD4+ lymphocytes which have considerable value in following HIV-infected individuals.
流式细胞术是确定亚群细胞群体的首选方法，需要精密的仪器设备和训练有素的操作人员。本文介绍了一种用于亚群测定的替代方法，该方法快速、简单，并且易于解读以得出绝对亚群计数。此方法是将抗体标记的微球添加到全血中，裂解后，使用普通光学显微镜在血细胞计数器上读取结果。检测总时间少于10分钟，其结果与使用流式细胞仪和血液分析仪计算绝对亚群值所获得的结果相当。本文针对在追踪HIV感染个体方面具有重要价值的CD4 +淋巴细胞展示了该方法。
