Viganò P, Gaffuri B, Ragni G, Di Blasio A M, Vignali M
II Department of Obstetrics and Gynecology, University of Milano, Italy.
J Clin Endocrinol Metab. 1997 Jan;82(1):101-5. doi: 10.1210/jcem.82.1.3646.
Some immune cellular components have been recently demonstrated to play a critical role in ovarian physiology. Resident ovarian white blood cells are known to produce cytokines that modulate granulosa cell (GC) functions and differentiation. Moreover, it has been postulated that, during the formation of the corpus luteum and luteolysis, human luteal cells are able to interact with lymphocytes and macrophages through some adhesion molecules. This study was designed to examine, at messenger RNA and protein levels, whether intercellular adhesion molecule (ICAM)-1, known to be involved in leukocyte-cell binding, is expressed by human GCs. Furthermore, we also investigated whether this molecule could be involved in the complex events that allow the interaction between the ovary and the immune system. GCs, obtained from women undergoing in vitro fertilization procedures, were enzymatically dispersed with collagenase and cultured for different time periods. To assess the presence of ICAM-1 messenger RNA, total RNA obtained from freshly aspirated GCs and GCs luteinized in culture was reverse transcribed and then amplified using two oligonucleotide primers specific for the human ICAM-1 gene. A single major DNA band of the expected size (943 bp) was obtained. The identity of this material with the human ICAM-1 sequence was further confirmed by restriction enzyme analysis. Surface ICAM-1 protein was detected by flow cytometric analysis on luteinized GCs cultured for 7 and 15 days. Finally, to evaluate a possible functional activity of ICAM-1, a 51Cr-release-binding assay between peripheral blood lymphocytes and luteinized GCs was performed in the presence and absence of a monoclonal antibody against ICAM-1. As a result, lymphocyte adhesion to GC monolayers was significantly, but not completely, inhibited by the anti-ICAM-1 monoclonal antibody. These findings demonstrate that intercellular interactions between GCs and the immune system are, at least in part, mediated by the adhesion molecule ICAM-1. Based on this data, we might speculate that this molecule could participate in the remodeling processes of the ovarian endocrine compartment.
最近有研究表明，一些免疫细胞成分在卵巢生理过程中发挥着关键作用。已知驻留于卵巢的白细胞会产生调节颗粒细胞（GC）功能和分化的细胞因子。此外，据推测，在黄体形成和黄体溶解过程中，人黄体细胞能够通过一些黏附分子与淋巴细胞和巨噬细胞相互作用。本研究旨在从信使核糖核酸（mRNA）和蛋白质水平检测已知参与白细胞-细胞结合的细胞间黏附分子（ICAM）-1是否由人颗粒细胞表达。此外，我们还研究了该分子是否参与卵巢与免疫系统之间相互作用的复杂过程。从接受体外受精手术的女性获取颗粒细胞，用胶原酶进行酶解分散，并培养不同时间段。为评估ICAM-1信使核糖核酸的存在情况，从新鲜吸出的颗粒细胞和培养中黄体化的颗粒细胞获取的总核糖核酸进行逆转录，然后使用针对人ICAM-1基因的两个寡核苷酸引物进行扩增。获得了一条预期大小（943碱基对）的单一主要DNA条带。通过限制性酶切分析进一步证实了该物质与人ICAM-1序列的一致性。通过流式细胞术分析检测培养7天和15天的黄体化颗粒细胞表面的ICAM-1蛋白。最后，为评估ICAM-1可能的功能活性，在有和没有抗ICAM-1单克隆抗体的情况下，进行外周血淋巴细胞与黄体化颗粒细胞之间的51铬释放结合试验。结果，抗ICAM-1单克隆抗体显著但未完全抑制淋巴细胞对颗粒细胞单层的黏附。这些发现表明，颗粒细胞与免疫系统之间的细胞间相互作用至少部分是由黏附分子ICAM-1介导的。基于这些数据，我们可以推测该分子可能参与卵巢内分泌区室的重塑过程。
