Aguirre Ghiso J, Diament M, D'elia I, Bal de Kier Joffé E, Klein S
Research Area, Institute of Oncology, Angel H. Roffo, University of Buenos Aires, Argentina.
Tumour Biol. 1997;18(1):41-52. doi: 10.1159/000218014.
We studied the effect of tumoral microenvironments on metastatic phenotypes. Therefore, murine mammary adenocarcinoma cells cultured in vivo in diffusion chambers (DC) were implanted intraperitoneally in BALB/c mice. The behavior of DC-cultured cells was compared with that of cells obtained from tumors growing subcutaneously or intraperitoneally and from primary cultures in vitro of the former. DC-cultured and control cells were inoculated into normal mice to evaluate their tumorigenicity and metastasizing ability. We found that DC-cultured cells were less tumorigenic and metastatic both in spontaneous and in experimental metastasis assays. The host response to tumor progression resulted in an early leukocytosis, probably due to the overproduction of a hematopoietic factor by the tumor cells. Finally, it was found that DC-cultured cells produced lower levels of urokinase-type plasminogen activator activity, while no differences were found in the metalloproteinase production compared to cells obtained from a tumor growing subcutaneously.
我们研究了肿瘤微环境对转移表型的影响。因此，将在扩散室（DC）中体内培养的小鼠乳腺腺癌细胞腹腔注射到BALB/c小鼠体内。将DC培养的细胞的行为与从皮下或腹腔内生长的肿瘤以及前者的体外原代培养物中获得的细胞的行为进行比较。将DC培养的细胞和对照细胞接种到正常小鼠中，以评估它们的致瘤性和转移能力。我们发现，在自发转移和实验性转移试验中，DC培养的细胞的致瘤性和转移性均较低。宿主对肿瘤进展的反应导致早期白细胞增多，这可能是由于肿瘤细胞过度产生造血因子所致。最后，发现DC培养的细胞产生的尿激酶型纤溶酶原激活剂活性水平较低，而与从皮下生长的肿瘤中获得的细胞相比，金属蛋白酶的产生没有差异。
