Monestier M
Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA.
Methods. 1997 Jan;11(1):36-43. doi: 10.1006/meth.1996.0385.
Individuals with systemic autoimmune diseases develop autoantibodies to nucleosome antigens. For many years, investigators have devoted much effort to precisely mapping epitopes on individual chromatin components. This approach, however, overlooks the existence of determinants that result from multimolecular interactions among nucleosome elements, such as DNA and histones. Anti-nucleosome antibodies can recognize a variety of complex epitopes and are especially prevalent in spontaneous and drug-induced lupus. Using numerous monoclonal anti-nucleosome antibodies obtained from autoimmune mice, we have further characterized these determinants and sequenced the variable region genes of these autoantibodies. I herein review these studies and their implications for the origin of antinuclear autoantibodies.
患有系统性自身免疫疾病的个体可产生针对核小体抗原的自身抗体。多年来，研究人员投入了大量精力来精确绘制单个染色质成分上的表位。然而，这种方法忽略了由核小体元件（如DNA和组蛋白）之间的多分子相互作用所产生的决定簇的存在。抗核小体抗体可识别多种复杂表位，尤其在自发性和药物性狼疮中普遍存在。利用从自身免疫小鼠获得的大量单克隆抗核小体抗体，我们进一步对这些决定簇进行了表征，并对这些自身抗体的可变区基因进行了测序。我在此回顾这些研究及其对抗核自身抗体起源的意义。
