Giles W, O'Callaghan S, Read M, Gude N, King R, Brennecke S
Discipline of Reproductive Medicine, School of Medicine and Health Sciences, University of Newcastle, Callaghan, Australia.
Obstet Gynecol. 1997 Jan;89(1):49-52. doi: 10.1016/s0029-7844(96)00371-7.
To assess the nitric oxide synthase activity in placentas from women with either normal or abnormal Doppler ultrasound umbilical artery flow velocity wave-forms who delivered by elective cesarean.
This prospective observational study involved 16 women admitted either for elective cesarean for standard obstetric indications (with normal umbilical artery Doppler waveform studies, n = 8) or with evidence of fetal or maternal complications of pregnancy (with abnormal umbilical artery Doppler studies, n = 8). Placental tissue was collected and frozen in liquid nitrogen immediately upon delivery. Following storage at -80C, nitric oxide synthase activity was analyzed by measuring the conversion of [3H]L-arginine to [3H]L-citrulline.
Placentas from women with abnormal umbilical artery flow velocity waveforms showed significantly lower mean nitric oxide synthase activity than did placentas from women with normal umbilical artery flow velocity wave-forms (Vmax = 11.3 pmol/minute/mg protein versus 22.6 pmol/minute/mg protein).
There is a statistically significant reduction in nitric oxide synthase activity in placentas from pregnancies with abnormal umbilical artery flow velocity waveforms.
评估择期剖宫产分娩的、脐动脉血流速度波形正常或异常的孕妇胎盘组织中一氧化氮合酶的活性。
这项前瞻性观察性研究纳入了16名孕妇，其中8名因标准产科指征行择期剖宫产（脐动脉多普勒波形研究正常），另外8名有胎儿或母亲妊娠并发症证据（脐动脉多普勒研究异常）。胎盘组织在分娩后立即采集并液氮冷冻。在-80°C储存后，通过测量[3H]L-精氨酸向[3H]L-瓜氨酸的转化来分析一氧化氮合酶活性。
脐动脉血流速度波形异常的孕妇胎盘组织中一氧化氮合酶的平均活性显著低于脐动脉血流速度波形正常的孕妇胎盘组织（最大反应速度分别为11.3 pmol/分钟/毫克蛋白和22.6 pmol/分钟/毫克蛋白）。
脐动脉血流速度波形异常的妊娠胎盘组织中，一氧化氮合酶活性有统计学意义的降低。
