新型白三烯受体拮抗剂ONO-1078对人外周血嗜酸性粒细胞的抑制作用

[Inhibitory effect of ONO-1078, a novel leukotriene receptor antagonist, on human peripheral blood eosinophils].

作者信息

Kobayashi S, Yoshida K, Mori A, Sugiyama H, Suko M, Okudaira H, Ito K, Inokuma S, Matsumura Y

机构信息

Department of Medicine and Physical Therapy, University of Tokyo.

出版信息

Arerugi. 1996 Nov;45(11):1166-71.

PMID:8990528

Abstract

To determine whether ONO-1078, leukotriene receptor antagonist, inhibits the activation of eosinophils, we investigated the effect of ONO-1078 on the release of leukotriene (LTC4) and eosinophil cationic protein (ECP) from human peripheral blood eosinophils of allergic patients and normal subjects. After preincubation with ONO-1078, eosinophils were incubated with calcium ionophore (A23187). ONO-1078 10(-5) and 10(-6) M inhibited LTC4 release of allergic patients eosinophils and 10(-5)-10(-9) M inhibited LTC4 release of normal subjects eosinophils. ONO-1078, 10(-5) M, also inhibited ECP release of eosinophils obtained from both asthma patients and normal subjects. We think that ONO-1078 may have another pharmacological effect to prevent the activation of human peripheral blood eosinophils by inhibiting LTC4 and ECP release. These results suggest that ONO-1078 might be useful in controlling bronchial asthma.

摘要

为了确定白三烯受体拮抗剂ONO - 1078是否能抑制嗜酸性粒细胞的活化，我们研究了ONO - 1078对过敏性患者和正常受试者外周血嗜酸性粒细胞中白三烯（LTC4）和嗜酸性粒细胞阳离子蛋白（ECP）释放的影响。在用ONO - 1078预孵育后，嗜酸性粒细胞与钙离子载体（A23187）一起孵育。10^(-5)和10^(-6) M的ONO - 1078抑制过敏性患者嗜酸性粒细胞的LTC4释放，10^(-5) - 10^(-9) M的ONO - 1078抑制正常受试者嗜酸性粒细胞的LTC4释放。10^(-5) M的ONO - 1078也抑制了哮喘患者和正常受试者嗜酸性粒细胞的ECP释放。我们认为ONO - 1078可能具有另一种药理作用，即通过抑制LTC4和ECP释放来防止人外周血嗜酸性粒细胞的活化。这些结果表明ONO - 1078可能有助于控制支气管哮喘。