巴西副球孢子菌重组27千道尔顿抗原蛋白的免疫学特性
Immunological characterization of a recombinant 27-kilodalton antigenic protein from Paracoccidioides brasiliensis.
作者信息
Ortiz B L, Garcia A M, Restrepo A, McEwen J G
机构信息
Molecular Biology Section, Corporación para Investigaciones Biológicas, Medellín, Colombia.
出版信息
Clin Diagn Lab Immunol. 1996 Mar;3(2):239-41. doi: 10.1128/cdli.3.2.239-241.1996.
Abstract
We report the expression in Escherichia coli of a 27-kDa antigenic protein from Paracoccidioides brasiliensis. When analyzed by immunoblotting, this recombinant antigenic protein was recognized by antibodies present in the sera of 40 of the 44 paracoccidioidomycosis patients studied. No cross-reactions were observed with sera from patients with other mycoses (histoplasmosis, aspergillosis, cryptococcosis, sporotrichosis, and chromoblastomycosis) or with tuberculosis.
摘要
我们报道了巴西副球孢子菌一种27 kDa抗原蛋白在大肠杆菌中的表达。通过免疫印迹分析，在所研究的44例副球孢子菌病患者中，有40例患者血清中的抗体可识别这种重组抗原蛋白。未观察到与其他真菌病（组织胞浆菌病、曲霉病、隐球菌病、孢子丝菌病和成芽细胞菌病）患者的血清或结核病患者的血清发生交叉反应。
相似文献
1
Immunological characterization of a recombinant 27-kilodalton antigenic protein from Paracoccidioides brasiliensis.巴西副球孢子菌重组27千道尔顿抗原蛋白的免疫学特性
Clin Diagn Lab Immunol. 1996 Mar;3(2):239-41. doi: 10.1128/cdli.3.2.239-241.1996.
2
Use of the 27-kilodalton recombinant protein from Paracoccidioides brasiliensis in serodiagnosis of paracoccidioidomycosis.巴西副球孢子菌27千道尔顿重组蛋白在副球孢子菌病血清诊断中的应用。
Clin Diagn Lab Immunol. 1998 Nov;5(6):826-30. doi: 10.1128/CDLI.5.6.826-830.1998.
3
A new Paracoccidioides brasiliensis 70-kDa heat shock protein reacts with sera from paracoccidioidomycosis patients.
Med Mycol. 2005 Sep;43(6):495-503. doi: 10.1080/13693780400029478.
4
Protective immunity induced by rPb27 of Paracoccidioides brasiliensis.巴西副球孢子菌rPb27诱导的保护性免疫。
Vaccine. 2008 Oct 9;26(43):5461-9. doi: 10.1016/j.vaccine.2008.07.097. Epub 2008 Aug 21.
5
Diagnosis of paracoccidioidomycosis by a dot blot assay using a recombinant Paracoccidioides brasiliensis p27 protein.使用重组巴西副球孢子菌p27蛋白通过斑点印迹法诊断副球孢子菌病
Mycoses. 2007 Jan;50(1):41-7. doi: 10.1111/j.1439-0507.2006.01306.x.
6
Identification of immunogenic proteins from Paracoccidioides brasiliensis antigenic fractions F0, FII and FIII.从巴西副球孢子菌抗原组分F0、FII和FIII中鉴定免疫原性蛋白。
Immunol Lett. 2005 Oct 15;101(1):24-31. doi: 10.1016/j.imlet.2005.04.007.
7
Heterologous expression, purification, and immunological reactivity of a recombinant HSP60 from Paracoccidioides brasiliensis.巴西副球孢子菌重组热休克蛋白60的异源表达、纯化及免疫反应性
Clin Diagn Lab Immunol. 2002 Mar;9(2):374-7. doi: 10.1128/cdli.9.2.374-377.2002.
8
Expression in bacteria of the gene encoding the gp43 antigen of paracoccidioides brasiliensis: immunological reactivity of the recombinant fusion proteins.巴西副球孢子菌gp43抗原编码基因在细菌中的表达：重组融合蛋白的免疫反应性
Clin Diagn Lab Immunol. 2002 Nov;9(6):1200-4. doi: 10.1128/cdli.9.6.1200-1204.2002.
9
Combined use of Paracoccidioides brasiliensis recombinant 27-kilodalton and purified 87-kilodalton antigens in an enzyme-linked immunosorbent assay for serodiagnosis of paracoccidioidomycosis.巴西副球孢子菌重组27千道尔顿抗原与纯化的87千道尔顿抗原联合用于酶联免疫吸附试验以进行副球孢子菌病的血清学诊断
J Clin Microbiol. 2003 Apr;41(4):1536-42. doi: 10.1128/JCM.41.4.1536-1542.2003.
10
The combined use of Paracoccidioides brasiliensis Pb40 and Pb27 recombinant proteins enhances chemotherapy effects in experimental paracoccidioidomycosis.巴西副球孢子菌 Pb40 和 Pb27 重组蛋白联合使用可增强实验性副球孢子菌病的化疗效果。
Microbes Infect. 2011 Nov;13(12-13):1062-72. doi: 10.1016/j.micinf.2011.06.004. Epub 2011 Jun 21.
引用本文的文献
1
Additive effect of rPb27 immunization and chemotherapy in experimental paracoccidioidomycosis.rPb27 免疫接种与化疗在实验性球孢子菌病中的相加效应。
PLoS One. 2011 Mar 10;6(3):e17885. doi: 10.1371/journal.pone.0017885.
2
Evidence for positive selection in putative virulence factors within the Paracoccidioides brasiliensis species complex.巴西副球孢子菌种复合体中假定毒力因子内正选择的证据。
PLoS Negl Trop Dis. 2008 Sep 17;2(9):e296. doi: 10.1371/journal.pntd.0000296.
3
Expression of antibodies directed to Paracoccidioides brasiliensis glycosphingolipids during the course of paracoccidioidomycosis treatment.巴西副球孢子菌糖鞘脂抗体在副球孢子菌病治疗过程中的表达
Clin Vaccine Immunol. 2007 Feb;14(2):150-6. doi: 10.1128/CVI.00285-06. Epub 2006 Nov 29.
4
Combined use of Paracoccidioides brasiliensis recombinant 27-kilodalton and purified 87-kilodalton antigens in an enzyme-linked immunosorbent assay for serodiagnosis of paracoccidioidomycosis.巴西副球孢子菌重组27千道尔顿抗原与纯化的87千道尔顿抗原联合用于酶联免疫吸附试验以进行副球孢子菌病的血清学诊断
J Clin Microbiol. 2003 Apr;41(4):1536-42. doi: 10.1128/JCM.41.4.1536-1542.2003.
5
Current status of nonculture methods for diagnosis of invasive fungal infections.侵袭性真菌感染非培养诊断方法的现状
Clin Microbiol Rev. 2002 Jul;15(3):465-84. doi: 10.1128/CMR.15.3.465-484.2002.
6
Use of the 27-kilodalton recombinant protein from Paracoccidioides brasiliensis in serodiagnosis of paracoccidioidomycosis.巴西副球孢子菌27千道尔顿重组蛋白在副球孢子菌病血清诊断中的应用。
Clin Diagn Lab Immunol. 1998 Nov;5(6):826-30. doi: 10.1128/CDLI.5.6.826-830.1998.
7
Identification and purification of specific Penicillium marneffei antigens and their recognition by human immune sera.马尔尼菲青霉菌特异性抗原的鉴定与纯化及其被人免疫血清识别的情况。
J Clin Microbiol. 1998 Apr;36(4):949-54. doi: 10.1128/JCM.36.4.949-954.1998.
本文引用的文献
1
Paracoccidioidomycosis: an update.副球孢子菌病：最新进展
Clin Microbiol Rev. 1993 Apr;6(2):89-117. doi: 10.1128/CMR.6.2.89.
2
Diagnosis of paracoccidioidomycosis by dot immunobinding assay for antibody detection using the purified and specific antigen gp43.采用纯化的特异性抗原gp43通过斑点免疫结合试验检测抗体来诊断副球孢子菌病。
J Clin Microbiol. 1994 Feb;32(2):554-6. doi: 10.1128/jcm.32.2.554-556.1994.
3
Growth of Paracoccidioides brasiliensis yeast phase in a chemically defined culture medium.巴西副球孢子菌酵母相在化学成分明确的培养基中的生长
J Clin Microbiol. 1980 Aug;12(2):279-81. doi: 10.1128/jcm.12.2.279-281.1980.
4
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
5
Predictive value of serologic tests in the diagnosis and follow-up of patients with paracoccidioidomycosis.
Rev Inst Med Trop Sao Paulo. 1987 Sep-Oct;29(5):276-83. doi: 10.1590/s0036-46651987000500003.
6
Identification of antigenic polypeptides of Paracoccidioides brasiliensis by immunoblotting.通过免疫印迹法鉴定巴西副球孢子菌的抗原多肽
J Med Vet Mycol. 1989;27(6):407-12.
7
Exocellular components of Paracoccidioides brasiliensis: identification of a specific antigen.巴西副球孢子菌的胞外成分：一种特异性抗原的鉴定
Infect Immun. 1986 Jul;53(1):199-206. doi: 10.1128/iai.53.1.199-206.1986.
8
Characterization of the cellular antigens of Paracoccidioides brasiliensis yeast form.
J Clin Microbiol. 1990 Jun;28(6):1188-93. doi: 10.1128/jcm.28.6.1188-1193.1990.
9
43-kilodalton glycoprotein from Paracoccidioides brasiliensis: immunochemical reactions with sera from patients with paracoccidioidomycosis, histoplasmosis, or Jorge Lobo's disease.巴西副球孢子菌的43千道尔顿糖蛋白：与副球孢子菌病、组织胞浆菌病或 Jorge Lobo 病患者血清的免疫化学反应
J Clin Microbiol. 1991 Aug;29(8):1610-5. doi: 10.1128/jcm.29.8.1610-1615.1991.
10
Isolation and antigenicity of a 45-kilodalton Paracoccidioides brasiliensis immunodominant antigen.巴西副球孢子菌45千道尔顿免疫显性抗原的分离与抗原性
Infect Immun. 1992 Jul;60(7):2667-71. doi: 10.1128/iai.60.7.2667-2671.1992.
Zotero 插件