Monaco F, Amati M, Visonà I
Clinica di Medicina del Lavoro, Università di Ancona, Ospedale. Regionale.
G Ital Med Lav. 1995 Jan-Nov;17(1-6):99-103.
We have verified that chemotaxis of isolated human polymorphonuclear leukocytes (PMN) was a target of in vitro toxicant effect of acrylonitrile (ACN). This toxicant induced a significant dose dependent decreasing of chemotaxis with 50% inhibition (IC50) occurring at 15 mM. We assume that PMN from workers exposed to ACN reacts vivo in a similar way to PMN exposed in vitro to ACN. We propose therefore to use chemotaxis assay as a biomarker of early biological effect of ACN in workers, since for their monitoring there are so far only internal dose indicators but no suitable effect indicators.
我们已经证实，分离出的人多形核白细胞（PMN）的趋化作用是丙烯腈（ACN）体外毒性作用的一个靶点。这种毒物可诱导趋化作用显著的剂量依赖性降低，在15 mM时出现50%的抑制（IC50）。我们假设，接触ACN的工人的PMN在体内的反应方式与体外接触ACN的PMN相似。因此，我们建议使用趋化试验作为ACN对工人早期生物学效应的生物标志物，因为迄今为止，对他们的监测仅有内剂量指标，而没有合适的效应指标。
