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[来自黄单胞菌属的溶菌酰胺酶的溶菌作用与革兰氏阳性菌细胞壁中靶标核糖醇磷壁酸的存在相关]

[Lytic action of lysoamidase from Xanthomonas sp. correlates with the presence of the target ribitol teichoic acids in the cell wall of gram-positive bacteria].

作者信息

Kulaev I S, Naumova I B, Streshinskaia G M, Tul'skaia E M, Stepnaia O A, Severin A I, Begunova E A

出版信息

Mikrobiologiia. 1996 May-Jun;65(3):326-32.

PMID:8992241
Abstract

Lysoamidase, a bacteriolytic complex from the culture liquid of Xanthomonas sp., hydrolyzed the cells walls of Staphylococcus aureus, Streptomyces chrysomallus, and Streptomyces azureus, which contain ribitol teichoic acids in addition to peptidoglycan. The cell walls of Streptomyces roseoflavus, Glycomyces harhinensis, and Nocardiopsis dassonvillei, containing glycerol teichoic acids, were not hydrolyzed by lysoamidase. The extent of the hydrolysis of 20-h Str. chrysomallus cells and cell walls, containing 40% ribitol teichoic acids, was considerably higher than that of 40-h cells and cell walls, containing 15% teichoic acids. Homogeneous bacteriolytic enzymes of the lysoamidase complex (muramidase and two bacteriolytic peptidases) most efficiently hydrolyzed S. aureus and Str. chrysomallus cell walls, characterized by the highest content of ribitol teichoic acids, and did not hydrolyze purified peptidoglycan.

摘要

溶菌酰胺酶是一种从黄单胞菌属培养液中提取的溶菌复合物,它能水解金黄色葡萄球菌、金色链霉菌和天蓝色链霉菌的细胞壁,这些细胞壁除了肽聚糖外还含有核糖醇磷壁酸。含有甘油磷壁酸的玫瑰黄链霉菌、哈氏糖霉菌和达松维尔拟诺卡氏菌的细胞壁未被溶菌酰胺酶水解。含有40%核糖醇磷壁酸的20小时培养的金色链霉菌细胞和细胞壁的水解程度明显高于含有15%磷壁酸的40小时细胞和细胞壁。溶菌酰胺酶复合物的纯溶菌酶(溶菌酶和两种溶菌肽酶)最有效地水解了以核糖醇磷壁酸含量最高为特征的金黄色葡萄球菌和金色链霉菌细胞壁,并且不水解纯化的肽聚糖。

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