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利用抗磷酸化波形蛋白和胶质纤维酸性蛋白抗体对单细胞中蛋白激酶活性进行可视化。

Visualization of protein kinase activities in single cells by antibodies against phosphorylated vimentin and GFAP.

作者信息

Inagaki N, Tsujimura K, Tanaka J, Sekimata M, Kamei Y, Inagaki M

机构信息

Department of Biochemistry, Aichi Open Center Research Institute, Japan.

出版信息

Neurochem Res. 1996 Jul;21(7):795-800. doi: 10.1007/BF02532302.

DOI:10.1007/BF02532302
PMID:8992382
Abstract

Vimentin and glial fibrillary acidic protein (GFAP) are intermediate filament proteins expressed in the cytoplasm of various types of cells. The head domains of these proteins are phosphorylated by various protein kinases. Site- and phosphorylation-specific antibodies which recognize a phosphorylated serine/threonine residue in the head domains and its flanking sequence provide a useful tool to monitor and visualize protein kinase activities in single cells.

摘要

波形蛋白和胶质纤维酸性蛋白（GFAP）是在各种类型细胞的细胞质中表达的中间丝蛋白。这些蛋白的头部结构域被各种蛋白激酶磷酸化。识别头部结构域及其侧翼序列中磷酸化丝氨酸/苏氨酸残基的位点特异性和磷酸化特异性抗体，为监测和可视化单细胞中的蛋白激酶活性提供了一种有用的工具。

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本文引用的文献

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Visualization and function of vimentin phosphorylation by cdc2 kinase during mitosis.有丝分裂过程中波形蛋白被细胞周期蛋白依赖性激酶2磷酸化的可视化及功能研究
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Identification of phosphorylation sites on glial fibrillary acidic protein for cdc2 kinase and Ca(2+)-calmodulin-dependent protein kinase II.确定胶质纤维酸性蛋白上细胞分裂周期蛋白2激酶和钙调蛋白依赖性蛋白激酶II的磷酸化位点。
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