[人降钙素和微型胰岛素原在细菌细胞中以与相应反义肽和金属结合肽形成的杂合蛋白形式进行生物合成]
[Biosynthesis of human calcitonin and mini-proinsulin in bacterial cells in the form of hybrid proteins with corresponding antisense peptides and a metal-binding peptide].
作者信息
Efimov V A, Buriakova A A, Fradkov A F, Chakhmakhcheva O G
出版信息
Bioorg Khim. 1996 Jul;22(7):503-9.
PMID:8992955
Abstract
To verify experimentally the molecular recognition theory, plasmids were constructed that provided the efficient synthesis of hybrid proteins composed of human calcitonin or miniproinsulin, the corresponding antisense peptides, and a histidine-rich metal-binding peptide. A method for isolation of the hybrid proteins by metal-chelating chromatography, cleavage, and renaturation was developed.
摘要
为了通过实验验证分子识别理论，构建了质粒，该质粒能够高效合成由人降钙素或微型胰岛素、相应的反义肽以及富含组氨酸的金属结合肽组成的杂合蛋白。开发了一种通过金属螯合色谱法分离杂合蛋白、进行切割和复性的方法。
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