Dumitrescu M, Zaharia C N, Baiu D, Barot-Ciorbaru R
Stefan S. Nicolau Institute of Virology, Bucharest, Romania.
Roum Arch Microbiol Immunol. 1995 Jan-Jun;54(1-2):43-51.
The effect of the "Nocardia lysozyme digest" (NLD) obtained from Nocardia opaca on the Epirubicine accumulation in erythrocytes was studied by flow cytometry. It was shown that the number of fluorescent cells increased under the NLD action due to the increased membrane permeability for epirubicine. An opposite effect was also recorded consisting in the decrease of the relative fluorescence intensity per cell. This effect was attributed to the fluorescence quenching by the oxygen metabolites produced by NDL. In this paper we present some results of the study of the NLD effect on the epirubicine (Epir) accumulation in the erythrocytes (E). It is shown by flow cytometry that the Epir accumulation in the red cells is modified by the presence of the NLD fraction.
采用流式细胞术研究了从不透明诺卡氏菌获得的“诺卡氏菌溶菌酶消化物”（NLD）对表柔比星在红细胞中蓄积的影响。结果表明，在NLD作用下，由于表柔比星的膜通透性增加，荧光细胞数量增加。还记录到了相反的效应，即每个细胞的相对荧光强度降低。这种效应归因于NDL产生的氧代谢产物导致的荧光猝灭。本文介绍了NLD对表柔比星（Epir）在红细胞（E）中蓄积影响的一些研究结果。流式细胞术表明，NLD组分的存在改变了红细胞中Epir的蓄积。
