神经肽对血管张力和环磷酸腺苷生成的影响。

The effect of neuropeptides on vessel tone and cAMP production.

作者信息

Yao W, Sheikh S P, Ottesen B, Jørgensen J C

机构信息

Department of Obstetrics and Gynecology, Hvidovre Hospital, University of Copenhagen, Denmark.

出版信息

Ann N Y Acad Sci. 1996 Dec 26;805:784-8. doi: 10.1111/j.1749-6632.1996.tb17557.x.

DOI:10.1111/j.1749-6632.1996.tb17557.x

PMID:8993477

Abstract

The effect of VIP, PHM, PHV, PACAP-27, and PACAP-38 on vessel tone and cAMP production was investigated in rabbit ovarian arteries in vitro. The peptides (10(-7)M) induced a significant relaxation on NA-precontracted vessels and displayed similar potencies. The cAMP accumulation induced by PACAP-27 and PACAP-38 was five times higher than the cAMP content induced by VIP, PHM, and PHV. NPY (10(-7)M) markedly reversed the relaxations induced by VIP, PHM, PHV, PACAP-27, and PACAP-38 but did not at all affect the cAMP production induced by these peptides. We conclude that the relaxation induced by VIP, PHM, PHV, PACAP-27, and PACAP-38 and the contraction evoked by NPY are not solely related to the changes of cAMP contents, and that in addition to cAMP, another intracellular signal transduction pathway may be involved.

摘要

在体外对兔卵巢动脉研究了血管活性肠肽（VIP）、肽组氨酸蛋氨酸（PHM）、肽组氨酸缬氨酸（PHV）、27 肽垂体腺苷酸环化酶激活肽（PACAP - 27）和 38 肽垂体腺苷酸环化酶激活肽（PACAP - 38）对血管张力和环磷酸腺苷（cAMP）生成的影响。这些肽（10⁻⁷M）对去甲肾上腺素（NA）预收缩的血管诱导出显著的舒张作用，且显示出相似的效力。PACAP - 27 和 PACAP - 38 诱导的 cAMP 积累比 VIP、PHM 和 PHV 诱导的 cAMP 含量高五倍。神经肽 Y（NPY，10⁻⁷M）显著逆转了 VIP、PHM、PHV、PACAP - 27 和 PACAP - 38 诱导的舒张作用，但对这些肽诱导的 cAMP 生成完全没有影响。我们得出结论，VIP、PHM、PHV、PACAP - 27 和 PACAP - 38 诱导的舒张作用以及 NPY 引起的收缩作用并非仅仅与 cAMP 含量的变化相关，并且除了 cAMP 之外，可能还涉及另一种细胞内信号转导途径。