Bodine-Fowler S C, Meyer R S, Moskovitz A, Abrams R, Botte M J
Department of Orthopaedics and Biomedical Sciences Graduate Program, UCSD School of Medicine and VA Medical Center, La Jolla, California, USA.
Muscle Nerve. 1997 Jan;20(1):29-37. doi: 10.1002/(sici)1097-4598(199701)20:1<29::aid-mus4>3.0.co;2-j.
The objectives of this study were 1) to determine the degree to which soleus motoneurons find their appropriate target following crush and transection injuries to the sciatic nerve, and 2) to determine whether repair of a transected nerve with a silicone tube leads to greater specificity of reinnervation and recovery of muscle function than the standard epineurial suture repair method. Sixty adult female Sprague-Dawley rats were randomly assigned to one of three sciatic nerve injury groups: crush injury, transection with epineurial suture repair, or transection with a silicone tube repair. The degree to which soleus motoneurons were able to find their appropriate target following a sciatic nerve injury was examined using a double labeling dye technique in which the original soleus motor pool was labeled with fast blue and reinnervating motoneurons were labeled with Dil. Soleus motoneurons were able to find their appropriate target following a crush injury. The accuracy of reinnervation following a transection injury and repair, however, was relatively poor. Only 14% of the original soleus motoneurons found the correct target following a transection injury. Repair of a lesioned nerve with a silicone tube and a 5-mm gap as opposed to epineurial sutures did not increase the specificity of reinnervation or the degree of muscle recovery.
1）确定在坐骨神经挤压伤和横断伤后比目鱼肌运动神经元找到其合适靶标的程度；2）确定用硅胶管修复横断神经是否比标准的神经外膜缝合修复方法能带来更高的神经再支配特异性和肌肉功能恢复程度。60只成年雌性Sprague-Dawley大鼠被随机分配到三组坐骨神经损伤组之一：挤压伤组、神经外膜缝合修复的横断伤组或硅胶管修复的横断伤组。采用双重标记染料技术检测坐骨神经损伤后比目鱼肌运动神经元找到其合适靶标的程度，其中用快蓝标记原始的比目鱼肌运动神经元池，用Dil标记再支配的运动神经元。比目鱼肌运动神经元在挤压伤后能够找到其合适靶标。然而，横断伤和修复后的神经再支配准确性相对较差。横断伤后只有14%的原始比目鱼肌运动神经元找到了正确靶标。与神经外膜缝合相比，用硅胶管修复有5毫米间隙的损伤神经并没有提高神经再支配的特异性或肌肉恢复程度。
