Zhang H, Haasch D, Idler K B, Okasinski G F
Department of Growth Factor Research, Abbott Laboratories, Abbott Park, IL 60064, USA.
Gene. 1996 Dec 12;183(1-2):1-6. doi: 10.1016/s0378-1119(96)00362-9.
B7-1 is one of the co-stimulatory factors which plays an important role in immunity. We have cloned and functionally mapped the promoter of the murine B7-1 gene using transient transfection assays in mouse L (tk-) cells. The B7-1 basal promoter consists of three positively regulated regions. The distal region, located at -2597 to -1555, contains an assortment of putative transcription factor binding sites. The proximal upstream region, located at -130 to -110, contains a tandem repeat sequence 5'-GTGTTCTAGTGTT-3'. A downstream region is positioned at +269 to +25. We have also identified an alternatively spliced form of the murine B7-1 gene in L (tk-) cells.
B7-1是共刺激因子之一，在免疫中发挥重要作用。我们利用小鼠L（tk-）细胞中的瞬时转染试验，克隆了小鼠B7-1基因的启动子并对其进行了功能定位。B7-1基础启动子由三个正向调节区域组成。远端区域位于-2597至-1555，包含各种假定的转录因子结合位点。近端上游区域位于-130至-110，包含串联重复序列5'-GTGTTCTAGTGTT-3'。一个下游区域位于+269至+25。我们还在L（tk-）细胞中鉴定出了小鼠B7-1基因的一种可变剪接形式。
