Stevens M K, Hassett D J, Radolf J D, Hansen E J
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235-9048, USA.
Gene. 1996 Dec 12;183(1-2):35-40. doi: 10.1016/s0378-1119(96)00417-9.
The sodC gene of Haemophilus ducreyi was cloned and sequenced. The deduced amino acid sequence of this protein exhibited 71.6% identity and 81.8% similarity to the H. influenzae and H. parainfluenzae copper (Cu), zinc (Zn)-superoxide dismutase (SOD) enzymes. This gene was localized to a 2.2-kb H. ducreyi chromosomal DNA insert in plasmid pHdSOD. SOD activity was expressed in cell-free extracts of Escherichia coli containing the recombinant plasmid pHdSOD and was localized to the periplasmic space. The Cu,Zn-SOD produced by the H. ducreyi sodC gene did not complement the aerobic growth defect of an E. coli SOD-deficient mutant.
克隆并测序了杜克雷嗜血杆菌的sodC基因。该蛋白推导的氨基酸序列与流感嗜血杆菌和副流感嗜血杆菌的铜（Cu）、锌（Zn）超氧化物歧化酶（SOD）酶具有71.6%的同一性和81.8%的相似性。该基因定位于质粒pHdSOD中2.2kb的杜克雷嗜血杆菌染色体DNA插入片段。SOD活性在含有重组质粒pHdSOD的大肠杆菌无细胞提取物中表达，并定位于周质空间。杜克雷嗜血杆菌sodC基因产生的Cu,Zn-SOD不能弥补大肠杆菌SOD缺陷突变体的需氧生长缺陷。
