一个与大肠杆菌prc具有同源性的新型枯草芽孢杆菌基因。
A new Bacillus subtilis gene with homology to Escherichia coli prc.
作者信息
Marasco R, Varcamonti M, Ricca E, Sacco M
机构信息
Dipartimento di Chimica, Università degli Studi di Salerno, Fisciano, (SA) Italy.
出版信息
Gene. 1996 Dec 12;183(1-2):149-52. doi: 10.1016/s0378-1119(96)00543-4.
We report the cloning of a 2-kb PstI-BamHI fragment of Bacillus subtilis DNA carrying an open reading frame of 1398 bp, herein designated orfRM1. This orf was shown to be transcribed only during vegetative growth from a putative sigma A-specific promoter. The deduced amino acid sequence predicted a polypeptide of 51 kDa (466 aa), which shows significant percentage of identity with the Escherichia coli Prc protein. However no Prc-like phenotypes were observed in a B. subtilis orfRM1 deletion-insertion mutant.
我们报道了枯草芽孢杆菌DNA中一个2 kb的PstI - BamHI片段的克隆，该片段携带一个1398 bp的开放阅读框，在此命名为orfRM1。已证明该开放阅读框仅在营养生长期间从一个假定的σA特异性启动子转录。推导的氨基酸序列预测为一个51 kDa（466个氨基酸）的多肽，它与大肠杆菌Prc蛋白具有显著的同源百分比。然而，在枯草芽孢杆菌orfRM1缺失插入突变体中未观察到类似Prc的表型。
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