Emoto A, Nakagawa M, Wakabayashi Y, Hanada T, Naito S, Nomura Y
Department of Urology and Neurosurgery, Oita Medical University, Japan.
J Urol. 1997 Feb;157(2):699-703.
To investigate the role of basic fibroblast growth factor (b-FGF) in angiogenesis in renal cell carcinoma.
We examined b-FGF expression in 25 patients with renal cell carcinoma (RCC) by reverse transcriptase polymerase chain reaction (RT-PCR). We measured the tube formation by human omental microvascular endothelial (HOME) cells induced by b-FGF in a three-dimensional collagen gel in the absence or presence of SN12C human renal cancer cells by means of computer-image analysis.
RT-PCR analysis detected b-FGF m-RNA in 20 (80%) of 25 patients with RCC. The tube formation of HOME cells was increased in the presence of SN12C cells overexpressing b-FGF mRNA, induced by exogenous b-FGF in a dose dependent manner and completely inhibited by anti-b-FGF antibody.
Basic-FGF may develop tumor angiogenesis by inducing microvessel tubulogenesis in patients with RCC.
探讨碱性成纤维细胞生长因子（b-FGF）在肾细胞癌血管生成中的作用。
我们通过逆转录聚合酶链反应（RT-PCR）检测了25例肾细胞癌（RCC）患者的b-FGF表达。我们借助计算机图像分析，在三维胶原凝胶中，于不存在或存在SN12C人肾癌细胞的情况下，测量了b-FGF诱导的人网膜微血管内皮（HOME）细胞的管形成。
RT-PCR分析在25例RCC患者中的20例（80%）检测到b-FGF信使核糖核酸。在过表达b-FGF信使核糖核酸的SN12C细胞存在的情况下，HOME细胞的管形成增加，外源b-FGF以剂量依赖方式诱导其增加，并被抗b-FGF抗体完全抑制。
碱性成纤维细胞生长因子可能通过诱导肾细胞癌患者的微血管管状生成来促进肿瘤血管生成。
