Slaninová M, Vlcková V, Brozmanová J, Morais M A, Henriques J A
Department of Genetics, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia.
Neoplasma. 1996;43(5):315-9.
RecA protein of E.coli is a multifunctional protein participating in genetic recombination, recombinational repair and mutagenesis. We used E.coli recA gene as a probe for complementation of repair defects after treatment of N-methyl-N'-nitro-N-nitrosoguanidine in the pso4-1 and rad51::URA3 mutants of S. cerevisiae. We tried to find the role of the RecA protein in S. cerevisiae mutants defective in different repair pathways. The RecA protein had no effect on survival of haploid and diploid pso4-1 mutants, but it had a significant effect on MNNG induced mutagenesis, which was increased to the wild type level. No effect of the RecA protein on survival was observed in rad51::URA3 mutant after MNNG treatment. However, in this case the RecA protein decreased the induced mutagenesis. We can suppose that the RecA protein, with its multifunctional enzymatic activity, can bind to special intermediates and initiate function of different repair pathways depending on repair defects of the mutants studied.
大肠杆菌的RecA蛋白是一种多功能蛋白，参与基因重组、重组修复和诱变。我们使用大肠杆菌recA基因作为探针，用于在酿酒酵母的pso4-1和rad51::URA3突变体中经N-甲基-N'-硝基-N-亚硝基胍处理后修复缺陷的互补实验。我们试图找出RecA蛋白在不同修复途径存在缺陷的酿酒酵母突变体中的作用。RecA蛋白对单倍体和二倍体pso4-1突变体的存活没有影响，但对MNNG诱导的诱变有显著影响，诱变率增加到野生型水平。在MNNG处理后，未观察到RecA蛋白对rad51::URA3突变体存活的影响。然而，在这种情况下，RecA蛋白降低了诱导的诱变率。我们可以推测，具有多功能酶活性的RecA蛋白可以结合特殊中间体，并根据所研究突变体的修复缺陷启动不同修复途径的功能。
