Blasko M, Chalupa I, Borovanský J, Toceková M, Siracký J
Department of Cytogenetics and Tumor Biology, Slovak Academy of Sciences, Bratislava, Slovakia.
Neoplasma. 1996;43(6):411-5.
The human melanoma B-HM8 cell line was derived from highly pigmented malignant skin melanoma. After 5 weeks of cultivation it entirely lost the pigmentation and has remained amelanotic since. Electron microscopy revealed neither premelanosomes nor melanosomes and the cells did not release detectable amount of dopa-oxidase activity into culture medium. Immunocytochemical studies using the polyclonal anti-S-100 antibody and detection of alpha-mannosidase activity in culture medium proved the melanoma origin of B-HM8 cells. Chromosomal changes in the karyotype of these cells were typical for human melanoma with chromosomes No. 1, 5, 7, 9, and 11 involved most frequently.
人黑色素瘤B-HM8细胞系源自高度色素沉着的恶性皮肤黑色素瘤。培养5周后，它完全失去色素沉着，此后一直无黑色素生成。电子显微镜检查未发现前黑素小体和黑素小体，且细胞未向培养基中释放可检测量的多巴氧化酶活性。使用多克隆抗S-100抗体进行的免疫细胞化学研究以及对培养基中α-甘露糖苷酶活性的检测证明了B-HM8细胞的黑色素瘤起源。这些细胞的核型中的染色体变化是人类黑色素瘤的典型特征，其中1、5、7、9和11号染色体最常受累。
