Fujii J, Higashi A, Nakano M
Department of Pharmaceutical Services, Kumamoto University Hospital, Japan.
Biol Pharm Bull. 1996 Dec;19(12):1614-7. doi: 10.1248/bpb.19.1614.
For analyzing the concentrations of drugs in hairs, a new method of digestion of hairs with Biopurase, a protease obtained from Bacillus subtilis, was examined. The concentrations of drugs in hairs were then determined in order to examine the usefulness of the protease for the digestion of hairs. The stability of five anticonvulsants in the protease solution was maintained over a 12-h period. In the clinical tests, the concentrations of the drugs in hairs obtained from patients who were taking anticonvulsants for a long time were determined. The concentration of phenobarbital in hairs in 10 patients taking phenobarbital ranged from 194 to 5020 ng/10 mg with a mean of 578 ng/10 mg, and the concentration of phenytoin in hairs in 6 patients taking phenytoin ranged from 44 to 299 ng/10 mg with a mean of 115 ng/10 mg. From these results, the transfer of phenobarbital and phenytoin from circulating blood into hairs was confirmed, and the usefulness of Biopurase for the digestion of hairs was proved.
为了分析毛发中的药物浓度，研究了一种用从枯草芽孢杆菌中获得的蛋白酶Biopurase消化毛发的新方法。然后测定毛发中的药物浓度，以检验该蛋白酶用于毛发消化的有效性。五种抗惊厥药在蛋白酶溶液中的稳定性在12小时内保持不变。在临床试验中，测定了长期服用抗惊厥药的患者毛发中的药物浓度。10名服用苯巴比妥的患者毛发中苯巴比妥的浓度范围为194至5020 ng/10 mg，平均为578 ng/10 mg；6名服用苯妥英的患者毛发中苯妥英的浓度范围为44至299 ng/10 mg，平均为115 ng/10 mg。从这些结果可以证实苯巴比妥和苯妥英从循环血液向毛发的转移，并证明了Biopurase用于毛发消化的有效性。
