Masauzi N
Third Department of Internal Medicine, Hokkaido University, School of Medicine, Sapporo, Japan.
Hokkaido Igaku Zasshi. 1996 Nov;71(6):771-83.
The expression of differentiation antigens (D/As) and adhesion molecules (A/Ms) was studied on CD34 positive (CD34+) cells in peripheral blood (PB) and bone marrow (BM) of patients treated with myelosuppressive chemotherapy followed by granulocyte colony-stimulating factor (G-CSF) administration (CT/G-CSF). CD33 positive rates of PB and BM CD34+ cells were significantly higher in the patients than those in the normal controls. CD49d positive rate of PB CD34+ was significantly lower than that of BM CD34+ cells in both the patients and the normal controls, and this decreased expression of CD49d on PB CD34+ cells was larger in the patients than in the normal controls. CD117 positive rate was significantly lower in PB CD34+ cells of the patients than that of their BM CD34+ cells and PB CD34+ cells of the normal control. These results suggested that more mature progenitor cells increased in the patients' PB and BM after CT/G-CSF, and that the decreased expression of CD49d played an important role on peripheralisation of CD34+ cells even in steady state, and the decreased expression of CD117 was related with the mobilization of CD34+ cells after CT/G-CSF. CT/G-CSF decreased the expression of CD117 and enhance the decrease of CD49d expression on PB CD34+ cells. These changes appeared to be involved in the mobilization mechanism of hematopoietic progenitor cells from BM into PB after CT/G-CSF.
对接受骨髓抑制化疗后给予粒细胞集落刺激因子（G-CSF）（CT/G-CSF）治疗的患者外周血（PB）和骨髓（BM）中CD34阳性（CD34+）细胞的分化抗原（D/As）和黏附分子（A/Ms）表达进行了研究。患者PB和BM中CD34+细胞的CD33阳性率显著高于正常对照。在患者和正常对照中，PB CD34+细胞的CD49d阳性率均显著低于BM CD34+细胞，且患者PB CD34+细胞上CD49d表达的降低幅度大于正常对照。患者PB CD34+细胞的CD117阳性率显著低于其BM CD34+细胞以及正常对照的PB CD34+细胞。这些结果表明，CT/G-CSF治疗后患者的PB和BM中更成熟的祖细胞增加，且即使在稳态下CD49d表达的降低对CD34+细胞的外周化也起重要作用，CD117表达的降低与CT/G-CSF后CD34+细胞的动员有关。CT/G-CSF降低了CD117的表达，并增强了PB CD34+细胞上CD49d表达的降低。这些变化似乎参与了CT/G-CSF后造血祖细胞从BM动员到PB的机制。
