Teofili L, Iovino M S, Sica S, Pierelli L, Menichella G, De Stefano V, Rumi C, Leone G
Department of Hematology, Catholic University of Sacred Heart, Rome, Italy.
Exp Hematol. 1994 Sep;22(10):990-5.
Peripheral blood (PB) mononuclear cells mobilized with chemotherapy and granulocyte colony-stimulating factor (G-CSF) were enriched in CD34+ cells; aliquots were seeded in long-term cultures (LTC) on bone marrow (BM)-derived stromal layers and in liquid cultures containing various growth factors. The final recovery of PB CD34+ cells was similar to normal BM controls, and no difference was found in the expression of CD33 and CD13 antigens; a lower number of CD34+/HLA-DR- cells was found in PB with respect to BM samples (p < 0.001). PB cells sustained hematopoiesis in LTC at least as long as BM cells. At week 3 and 4, PB total mononuclear cell (MC) and CD34(+)-selected cell cultures showed a higher nonadherent cell recovery compared to the respective BM controls (p = 0.05 and p < 0.01). The liquid culture of PB CD34+ cells in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3), and stem cell factor (SCF) resulted in a marked and long-lasting increase of colony-forming units-granulocyte/macrophage (CFU-GM). Taken together, our data suggest that chemotherapy and G-CSF-primed cells contain a considerable number of both committed and early precursors, accounting for the rapid hematopoietic recovery observed after their reinfusion following myeloablative chemotherapy.
经化疗和粒细胞集落刺激因子（G-CSF）动员的外周血（PB）单个核细胞富含CD34+细胞；将等分试样接种于骨髓（BM）来源的基质层上进行长期培养（LTC），并接种于含有各种生长因子的液体培养基中。PB CD34+细胞的最终回收率与正常BM对照相似，且CD33和CD13抗原的表达未发现差异；与BM样本相比，PB中CD34+/HLA-DR-细胞数量较少（p<0.001）。PB细胞在LTC中维持造血的时间至少与BM细胞相同。在第3周和第4周，PB总单个核细胞（MC）和CD34(+)-分选细胞培养物的非贴壁细胞回收率高于各自的BM对照（p=0.05和p<0.01）。在粒细胞-巨噬细胞集落刺激因子（GM-CSF）、白细胞介素-3（IL-3）和干细胞因子（SCF）存在的情况下，PB CD34+细胞的液体培养导致集落形成单位-粒细胞/巨噬细胞（CFU-GM）显著且持久地增加。综上所述，我们的数据表明，经化疗和G-CSF预处理的细胞含有相当数量的定向祖细胞和早期祖细胞，这解释了在清髓性化疗后回输这些细胞后观察到的快速造血恢复现象。
