使用阴离子交换高效液相色谱法检测血红蛋白变异体以诊断β地中海贫血及其他血红蛋白病。
Detection of haemoglobin variants for the diagnosis of beta thalassaemia & other haemoglobinopathies using anion exchange high performance liquid chromatography.
作者信息
Rao V B, Bannerjee M K, Shewale L H, Panthaki M H, Bandodkar S B
机构信息
Haematology Department, Research Society, B.J. Wadia Hospital for Children, Mumbai.
出版信息
Indian J Med Res. 1996 Dec;104:365-73.
We describe the precise identification, separation and quantitation of several haemoglobin (Hb) variants such as Hbs A2, A, F, S, E, J, H, D-Punjab and Q-India in untransfused patients with beta thalassaemia and other haemoglobinopathies prevalent in the Indian population using whole blood (5 microliters), red cell lysates (2 microliters) or dried blood specimens on filter paper, using a conventional anion exchange high performance liquid chromatography (AX-HPLC) system. Samples were simultaneously studied for the presence of haemoglobin variants by alkaline electrophoresis. The problems in analysing rare variants and separating the closely migrating variants, encountered in the conventional methods could be resolved using the HPLC technique.
我们描述了使用全血（5微升）、红细胞裂解液（2微升）或滤纸上的干血标本，通过传统的阴离子交换高效液相色谱（AX-HPLC）系统，对未输血的β地中海贫血患者及印度人群中普遍存在的其他血红蛋白病患者的几种血红蛋白（Hb）变体，如Hb A2、A、F、S、E、J、H、D-旁遮普和Q-印度，进行精确鉴定、分离和定量。通过碱性电泳同时研究样本中血红蛋白变体的存在情况。使用HPLC技术可以解决传统方法中分析罕见变体和分离迁移相近变体时遇到的问题。
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