Ibrahim A, Liesack W, Steigerwalt A G, Brenner D J, Stackebrandt E, Robins-Browne R M
Department of Microbiology, University of Queensland, Brisbane, Australia.
FEMS Microbiol Lett. 1997 Jan 1;146(1):73-8. doi: 10.1111/j.1574-6968.1997.tb10173.x.
Thirty-eight bacterial isolates from raw milk samples in Queensland, Australia were identified as members of the genus Yersinia on the basis of biochemical profile, ability to hybridize with a genus-specific DNA probe, comparative 16S rDNA sequence analysis, and the presence of characteristic 16S rDNA signature nucleotides which occur in all Yersinia spp. Twenty-five of these isolates reacted with typing sera (O:22 or O:58) of Y. enterocolitica; the remainder were non-typable. None of the isolates displayed any of the phenotypic or genetic virulence-associated characteristics of Y. enterocolitica. Comparative 16S rDNA sequence analysis revealed that members of this group appear to represent a new sub-line within the genus Yersinia, most closely related to Y. frederiksenii hybridization group 2 (unnamed genomospecies 2). This findings was confirmed by DNA hybridization studies which indicated that the strains belonged to the unnamed genomospecies, Yersinia frederiksenii genomospecies 2, which is biochemically indistinguishable from Y. frederiksenii (Y. frederiksenii genomospecies 1). A 23-nucleotide 16S rDNA signature stretch which characterised these strains was identified.
基于生化特征、与属特异性DNA探针杂交的能力、比较16S rDNA序列分析以及所有耶尔森氏菌属物种中均存在的特征性16S rDNA特征核苷酸，从澳大利亚昆士兰州的生乳样本中分离出的38株细菌被鉴定为耶尔森氏菌属成员。其中25株分离株与小肠结肠炎耶尔森氏菌的分型血清（O:22或O:58）发生反应；其余的无法分型。所有分离株均未表现出小肠结肠炎耶尔森氏菌的任何表型或遗传毒力相关特征。比较16S rDNA序列分析表明，该组成员似乎代表耶尔森氏菌属内的一个新亚系，与弗氏耶尔森氏菌杂交组2（未命名基因组种2）关系最为密切。DNA杂交研究证实了这一发现，表明这些菌株属于未命名基因组种，即弗氏耶尔森氏菌基因组种2，其生化特征与弗氏耶尔森氏菌（弗氏耶尔森氏菌基因组种1）无法区分。鉴定出了一段23个核苷酸的16S rDNA特征序列，该序列可表征这些菌株。
