Bucholski K A, Begerow J, Winneke G, Dunemann L
Medizinisches Institut für Umwelthygiene, Department of Analytical Chemistry, Düsseldorf, Germany.
J Chromatogr A. 1996 Nov 22;754(1-2):479-85. doi: 10.1016/s0021-9673(96)00344-5.
A fast and reliable method for the determination of polychlorinated biphenyls (PCBs) and chlorinated pesticides in human body fluids and tissues is presented. Sample clean-up and selective enrichment of analytes are carried out by liquid column chromatography without a prior solvent extraction step. Analytes are determined either by high-resolution capillary gas chromatography with mass spectrometric detection, or by dual-column GC followed by electron-capture detection. A procedure is described for the simultaneous determination of environmental levels of the PCB congeners 28, 52, 101, 138, 153 and 180 in human cord serum. The method allows the simultaneous determination of chlorinated pesticides such as aldrin, DDT, DDE, BHC and HCB and is also applicable to other biological matrices, such as bone marrow or tissues.
本文介绍了一种快速、可靠的测定人体体液和组织中多氯联苯（PCBs）及氯代农药的方法。样品净化和分析物的选择性富集通过液相柱色谱法进行，无需事先进行溶剂萃取步骤。分析物可通过带质谱检测的高分辨率毛细管气相色谱法测定，或通过双柱气相色谱法随后进行电子捕获检测来测定。文中描述了一种同时测定人脐带血清中PCB同系物28、52、101、138、153和180环境水平的方法。该方法可同时测定氯代农药，如艾氏剂、滴滴涕、滴滴伊、六六六和六氯苯，也适用于其他生物基质，如骨髓或组织。
