Rupcić J, Blagović B, Marić V
Department of Chemistry and Biochemistry, Faculty of Medicine, University of Rijeka, Croatia.
J Chromatogr A. 1996 Nov 29;755(1):75-80. doi: 10.1016/s0021-9673(96)00579-1.
Candida lipolytica yeast, grown on 1% methanol as the only carbon and energy source, synthesized 4.9% of dry cell mass as lipids, 52.3% of which were polar lipids. Polar lipids consisted mainly of phospholipids and sphingolipids as their minor components. The total long-chain bases content has been found to account only for 0.7% of the polar lipids. The long-chain bases composition determined by thin-layer and gas chromatography shows a preponderance of trihydroxy bases and a small amount of dihydroxy bases. The striking finding was the high content of 19-phytosphingosine (90.8% of total long-chain bases). Fatty acid (FA) composition of polar lipids was characterized by the relatively high concentration of unsaturated fatty acids (66.4% of total FA) and by the predominance of fatty acids with 16 carbon atoms (85.0% of total FA).
解脂耶氏酵母在以1%甲醇作为唯一碳源和能源的条件下生长，合成的脂质占干细胞质量的4.9%，其中52.3%为极性脂质。极性脂质主要由磷脂组成，鞘脂为次要成分。已发现长链碱基总量仅占极性脂质的0.7%。通过薄层色谱和气相色谱测定的长链碱基组成显示，三羟基碱基占优势，二羟基碱基含量较少。显著的发现是19-植物鞘氨醇含量很高（占长链碱基总量的90.8%）。极性脂质的脂肪酸（FA）组成特点是不饱和脂肪酸浓度相对较高（占总FA的66.4%），且16个碳原子的脂肪酸占优势（占总FA的85.0%）。
