Alliangana D M
Department of Bioscience and Biotechnology, University of Strathclyde, Glasgow, Scotland, U.K.
East Afr Med J. 1996 Nov;73(11):752-7.
Exposure of BHK-21 (baby hamster kidney cells) to varying concentration of beta-carotene (0- 156 nM) induced a reduction in a cell proliferation rate, increased lipid peroxidation breakdown products, elevated levels of cellular reduced glutathione (GSH), and increased cellular DNA content. Similarly concentration of hydrogen peroxide (0-1.0 microM) added to growth medium truncated rates of cell proliferation as hydrogen peroxide (H2O2) concentration increased and augmented cellular DNA content dramatically. Electrophoresis analysis of DNA from cells treated with H2O2 on agarose gel indicated no excessive DNA fragmentation. Escalating concentration of flavonoid quercitin (0-10 microM) in culture cell medium enhanced the rate of cell growth and diminished levels of lipid peroxidation breakdown products. Quinacrine an inhibitor of phospholipase A2 activity not only abated the decline in cellular lipid peroxidation breakdown products but also abridged the rate of cell proliferation. A combination of beta-carotene (50 nM) and H2O2 (100 microM) added to growth medium depressed cell growth further and increased cellular lipid peroxidation breakdown products. High levels of lipid peroxidation breakdown products were also evident in newly established cultures but these levels decreased with increased growth rate.
将BHK - 21（幼仓鼠肾细胞）暴露于不同浓度的β-胡萝卜素（0 - 156 nM）会导致细胞增殖速率降低、脂质过氧化分解产物增加、细胞内还原型谷胱甘肽（GSH）水平升高以及细胞DNA含量增加。同样，向生长培养基中添加不同浓度的过氧化氢（0 - 1.0 microM），随着过氧化氢（H2O2）浓度的增加，细胞增殖速率降低，且细胞DNA含量显著增加。对用H2O2处理的细胞的DNA在琼脂糖凝胶上进行电泳分析表明没有过度的DNA片段化。在培养基中增加黄酮类槲皮素的浓度（0 - 10 microM）可提高细胞生长速率并降低脂质过氧化分解产物的水平。磷脂酶A2活性抑制剂奎纳克林不仅减轻了细胞脂质过氧化分解产物的下降，还降低了细胞增殖速率。向生长培养基中添加β-胡萝卜素（50 nM）和H2O2（100 microM）的组合进一步抑制细胞生长并增加细胞脂质过氧化分解产物。在新建立的培养物中也明显存在高水平的脂质过氧化分解产物，但这些水平随着生长速率的增加而降低。
