Watanabe A, Mishima H, Kotsimbos T C, Hojo M, Renzi P M, Martin J G, Hamid Q A
Meakins-Christie Laboratories, McGill University, Montreal, Quebec, Canada.
Am J Respir Cell Mol Biol. 1997 Jan;16(1):69-74. doi: 10.1165/ajrcmb.16.1.8998081.
Late allergic airway responses can be transferred by CD4+ T cells in the rat. To investigate the role of T-cell cytokines in these responses, we examined the expression of mRNA for Th2 (interleukin [IL]-4 and IL-5) and Th1 (IL-2 and interferon gamma [INF-gamma])-type cytokines in Brown Norway rats that were administered either antigen-primed W3/25(CD4)+ or OX8(CD8)+ T cells. Donors were actively sensitized by subcutaneous injection of ovalbumin (OVA) in the neck and T cells were obtained from the cervical lymph nodes by immunomagnetic cell sorting for administration to unsensitized rats. Control rats received bovine serum albumin (BSA)-primed CD4+ and CD8+ T cells. Two days later, recipient rats were challenged with aerosolized OVA, and bronchoalveolar lavage (BAL) was performed 8 h after challenge. BAL cells expressing mRNA for IL-2, IL-4, IL-5, and INF-gamma were analyzed using the technique of in situ hybridization. Recipients of OVA-primed CD4+ T cells had an increase in the fraction of BAL cells expressing mRNA for IL-4 and IL-5 compared with BSA-primed CD4+ or OVA-primed CD8+ cells (P < 0.001). Recipients of CD8+ T cells had an increase in INF-gamma mRNA expression after OVA challenge compared with recipients of BSA-primed-CD8+ or OVA-primed CD4+ T cells (P < 0.001). In conclusion, T-cell-dependent allergen-induced late responses are associated with the expression of mRNA for IL-4 and IL-5, indicating Th2 cell activation. Furthermore, the increased expression of INF-gamma in allergen challenge recipients of antigen-primed CD8+ T cells suggests that CD8+ T cells may be important in modulating allergic responses.
迟发型变应性气道反应可由大鼠的CD4+ T细胞传递。为研究T细胞细胞因子在这些反应中的作用，我们检测了经抗原致敏的W3/25(CD4)+或OX8(CD8)+ T细胞处理的棕色挪威大鼠中Th2（白细胞介素[IL]-4和IL-5）和Th1（IL-2和干扰素γ[INF-γ]）型细胞因子mRNA的表达。供体通过颈部皮下注射卵清蛋白（OVA）进行主动致敏，通过免疫磁珠细胞分选从颈部淋巴结获取T细胞，用于给未致敏大鼠注射。对照大鼠接受牛血清白蛋白（BSA）致敏的CD4+和CD8+ T细胞。两天后，受体大鼠用雾化OVA激发，激发后8小时进行支气管肺泡灌洗（BAL）。使用原位杂交技术分析表达IL-2、IL-4、IL-5和INF-γ mRNA的BAL细胞。与BSA致敏的CD（4）+或OVA致敏的CD8+细胞相比，OVA致敏的CD4+ T细胞受体中表达IL-4和IL-5 mRNA的BAL细胞比例增加（P<0.001）。与BSA致敏的CD8+或OVA致敏的CD4+ T细胞受体相比，OVA激发后CD8+ T细胞受体中INF-γ mRNA表达增加（P<0.001）。总之，T细胞依赖性变应原诱导的迟发反应与IL-4和IL-5 mRNA的表达相关，表明Th2细胞被激活。此外，抗原致敏的CD8+ T细胞激发受体中INF-γ表达增加表明CD8+ T细胞在调节变应性反应中可能起重要作用。
