Koyama T, Terauchi K
Central Research Institute, Nippon Paint Co. Ltd., Osaka, Japan.
J Chromatogr B Biomed Appl. 1996 Apr 26;679(1-2):31-40. doi: 10.1016/0378-4347(96)00006-0.
A preparation method of a novel type of packing materials for high-performance liquid affinity chromatography to determine glycated proteins was studied, and a fundamental study on its application to diabetic serum was conducted. To quantify glycated proteins such as glycated serum albumin, a new hydrophilic and durable porous polymer particle recently developed in our laboratory was used as the basic matrix. The matrix was activated with 1,1'-carbonyldiimidazole (CDI), and optimization of the coupling reaction between these CDI-activated matrix and m-aminophenylboronic acid hemisulfate (APBA) as affinity ligand was investigated. The optimum value for the APBA coupling yield was found to be obtained under acidic conditions very different from the data reported by previous workers. Using this APBA-immobilized matrix an affinity column was prepared, and its usefulness in HPLC separation of glycated serum proteins was investigated. Also, the fundamental and preliminary results for diagnosis of diabetes mellitus are discussed in this paper.
研究了一种用于高效液相亲和色谱测定糖化蛋白的新型填充材料的制备方法，并对其在糖尿病血清中的应用进行了基础研究。为了定量糖化血清白蛋白等糖化蛋白，使用了我们实验室最近开发的一种新型亲水性耐用多孔聚合物颗粒作为基本基质。用1,1'-羰基二咪唑（CDI）对基质进行活化，并研究了这些CDI活化基质与作为亲和配体的间氨基苯硼酸半硫酸盐（APBA）之间偶联反应的优化。发现APBA偶联产率的最佳值是在与先前工作者报道的数据非常不同的酸性条件下获得的。使用这种固定化APBA的基质制备了亲和柱，并研究了其在高效液相色谱分离糖化血清蛋白中的实用性。此外，本文还讨论了糖尿病诊断的基础和初步结果。
