在酿酒酵母中决定磺酰脲类甲基抗性的SMR1新等位基因的分子克隆。
Molecular cloning of a novel allele of SMR1 which determines sulfometuron methyl resistance in Saccharomyces cerevisiae.
作者信息
Xie Q, Jiménez A
机构信息
Centro de Biología Molecular Severo Ochoa (UAM/CSIC), Universidad Autónoma, Canto Blanco, Madrid, Spain.
出版信息
FEMS Microbiol Lett. 1996 Apr 1;137(2-3):165-8. doi: 10.1111/j.1574-6968.1996.tb08100.x.
A different mutation (SMR1B) to SMR1-410 (ilv2), which determines resistance to sulfometuron methyl in Saccharomyces cerevisiae, was cloned by PCR. Sequence analysis indicated a C to T change at position 575 of the ILV2 coding sequence, which results in a proline transition to leucine at position 192. Similarly to SMR1-410, the SMR1B gene was confirmed as a convenient dominant selective marker for yeast transformation including industrial strains.
通过PCR克隆了酿酒酵母中一种与决定对甲磺隆抗性的SMR1 - 410（ilv2）不同的突变（SMR1B）。序列分析表明，ILV2编码序列第575位发生了C到T的变化，导致第192位的脯氨酸转变为亮氨酸。与SMR1 - 410类似，SMR1B基因被确认为用于酵母转化（包括工业菌株）的便捷显性选择标记。
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