在四种代表性口腔兼性厌氧菌中鉴定出新型FNR同源物：赭色二氧化碳嗜纤维菌、产唾二氧化碳嗜纤维菌、嗜沫嗜血杆菌和伴放线放线杆菌。
Novel FNR homologues identified in four representative oral facultative anaerobes: Capnocytophaga ochracea, Capnocytophaga sputigena, Haemophilus aphrophilus, and Actinobacillus actinomycetemcomitans.
作者信息
Hattori T, Takahashi K, Nakanishi T, Ohta H, Fukui K, Taniguchi S, Takigawa M
机构信息
Department of Biochemistry and Molecular Dentistry, Okayama University Dental School, Japan.
出版信息
FEMS Microbiol Lett. 1996 Apr 1;137(2-3):213-20. doi: 10.1111/j.1574-6968.1996.tb08108.x.
Based upon DNA sequence data and positive immunochemical reactivity of expressed protein, novel homologues of the FNR family were identified in four representative oral facultative anaerobes: Capnocytophaga ochracea, Capnocytophaga sputigena, Haemophilus aphrophilus, and Actinobacillus actinomycetemcomitans. The similarity to E. coli FNR and to HlyX (itself 71% similar to E. coli FNR, while regulating expression of hemolysin operon in Actinobacillus pleuropneumoniae) was estimated from the deduced partial amino acid sequence to be, in the above order of tested species, 98, 98, 86, and 85%, and 75, 75, 88, and 88%, respectively. The phylogenetic relatedness indicates a rather closer link of HlyX to the FNR homologues from both pathogens, H. aphrophilus and A. actinomycetemcomitans. The possibility that the A. actinomycetemcomitans FNR homologue functions as a redox-sensing transcriptional factor to regulate, in addition to anaerobic respiration, microaerobic expression of the leukotoxin operon (ltx gene) is suggested.
基于DNA序列数据以及表达蛋白的阳性免疫化学反应，在四种代表性口腔兼性厌氧菌中鉴定出了FNR家族的新同源物：赭色纤毛菌、产唾纤毛菌、嗜沫嗜血杆菌和伴放线放线杆菌。根据推导的部分氨基酸序列估计，与大肠杆菌FNR以及HlyX（其本身与大肠杆菌FNR有71%的相似性，同时调节胸膜肺炎放线杆菌中溶血素操纵子的表达）的相似性，按上述测试物种的顺序分别为98%、98%、86%和85%，以及75%、75%、88%和88%。系统发育相关性表明，HlyX与来自两种病原体嗜沫嗜血杆菌和伴放线放线杆菌的FNR同源物有更紧密的联系。有人提出，伴放线放线杆菌FNR同源物可能作为一种氧化还原感应转录因子，除了调节无氧呼吸外，还调节白细胞毒素操纵子（ltx基因）的微需氧表达。
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