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细胞冷冻保存进一步改进的一些方面。

Some aspects for the further improvement in cell cryopreservation.

作者信息

Hackensellner H A, Matthes G

出版信息

Folia Haematol Int Mag Klin Morphol Blutforsch. 1979;106(3):389-93.

PMID:89990
Abstract

According to theoretical considerations and to results of own experimental investigations in heart muscle fragments of adult rats the authors are of opinion that more attention should be attributed to the cryoprotective medium as a whole, i.e. not only to the proper cryoprotectant, but to the other components of the cryoprotective medium as well. The suppose that the still unsatisfactory results of cell preservation, including blood and bone marrow cells, can be essentially improved by optimizing the composition of the basal medium.

摘要

根据理论考量以及对成年大鼠心肌片段进行的自身实验研究结果,作者认为应更多地关注整个冷冻保护介质,即不仅要关注合适的冷冻保护剂,还要关注冷冻保护介质的其他成分。他们推测,通过优化基础培养基的成分,包括血液和骨髓细胞在内的细胞保存效果仍不尽人意的情况可得到实质性改善。

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