Jonscher K R, Yates J R
Department of Molecular Biotechnology, University of Washington, Seattle 98195-7730, USA.
Anal Chem. 1996 Feb 15;68(4):659-67. doi: 10.1021/ac950978s.
A hybrid tandem mass spectrometer is constructed by interfacing a quadrupole mass filter (Q) to a quadrupole ion trap mass spectrometer (QITMS) and is evaluated for the analysis of mixtures. The mass filter is set to selectively inject ions of a particular m/z or, in scanning mode, to sequentially inject ions into the QITMS for subsequent manipulation and detection. Performance of the instrument is demonstrated using a mixture of ions created by electron impact ionization of perfluorotributylamine (FC-43) and peptide ions generated by pulsed Cs+ bombardment. Resulting data are compared to those obtained by utilizing only the ion trap. Molecular weight, fragmentation, and high-resolution analyses for the sequentially injected mass-filtered peptides show improved performance over similar measurements employing only the ion trap mass spectrometer. Performance is optimized when ions are not rf-isolated in the QITMS. Using the hybrid, a resolution of 33,200 is achieved for angiotensin I. Dramatic reduction of space charge-induced signal suppression is demonstrated for LSIMS of Glu-fibrinopeptide B. 'On-the-fly' collision-induced dissociation is performed for m/z 502 from FC-43, where fragmentation is induced by increasing the ion injection energy. Collision-induced dissociation efficiencies for fragmentation of angiotensin I by resonance excitation are investigated as a function of cooling time for different modes of operation of the hybrid. A current limitation of the instrument is the time required to port the data for acquisition.
通过将四极杆质量过滤器（Q）与四极杆离子阱质谱仪（QITMS）相连构建了一台混合串联质谱仪，并对其进行混合物分析评估。质量过滤器设置为选择性注入特定质荷比（m/z）的离子，或者在扫描模式下，将离子依次注入QITMS进行后续处理和检测。使用全氟三丁胺（FC - 43）电子轰击电离产生的离子混合物以及脉冲Cs⁺轰击产生的肽离子来展示该仪器的性能。将所得数据与仅使用离子阱获得的数据进行比较。对依次注入的经过质量过滤的肽进行分子量、碎片分析和高分辨率分析，结果表明其性能优于仅使用离子阱质谱仪进行的类似测量。当离子在QITMS中不进行射频隔离时，性能得到优化。使用该混合仪器，血管紧张素I的分辨率达到33,200。对于Glu - 纤维蛋白肽B的液相二次离子质谱（LSIMS），证明了空间电荷诱导的信号抑制显著降低。对FC - 43的m/z 502进行“飞行中”碰撞诱导解离，通过增加离子注入能量来诱导碎片形成。研究了通过共振激发使血管紧张素I碎片化的碰撞诱导解离效率与混合仪器不同操作模式下冷却时间的函数关系。该仪器目前的一个限制是传输数据进行采集所需的时间。
