Peterson P, Salmi H, Hyöty H, Miettinen A, Ilonen J, Reijonen H, Knip M, Akerblom H K, Krohn K
Institute of Medical Technology and University Hospital, University of Tampere, Finland.
Clin Immunol Immunopathol. 1997 Jan;82(1):37-42. doi: 10.1006/clin.1996.4277.
We have studied the sera from 304 patients with insulin-dependent diabetes mellitus (IDDM) for steroid 21-hydroxylase (P450c21) autoantibodies by an in vitro translation and immunoprecipitation assay. Autoantibodies to P450c21 were found in 7 patients with IDDM (2.3%). When the IDDM patients with P450c21 antibodies were analyzed for their HLA, 6 of them (86%) belonged to the HLA DQB1*0201-positive group. A strong correlation (r = 0.91, P < 0.001) of immunoprecipitation results was observed with adrenocortical autoantibodies detected by indirect immunofluorescence, indicating that the adrenal autoantibodies in patients with IDDM were anti-P450c21 autoantibodies. The levels of P450c21 autoantibodies were significantly higher (P < 0.05) in patients with IDDM than in 13 (9 P450c21 autoantibody positive) patients with Addison's disease. Although the occurrence of P450c21 antibodies in IDDM was relatively low, antibody-positive patients had high antibody levels and may have an ongoing subclinical process of adrenal autoimmunity.
我们通过体外翻译和免疫沉淀试验，研究了304例胰岛素依赖型糖尿病（IDDM）患者血清中的类固醇21-羟化酶（P450c21）自身抗体。在7例IDDM患者（2.3%）中发现了P450c21自身抗体。对携带P450c21抗体的IDDM患者进行HLA分析时，其中6例（86%）属于HLA DQB1*0201阳性组。观察到免疫沉淀结果与间接免疫荧光检测的肾上腺皮质自身抗体有很强的相关性（r = 0.91，P < 0.001），这表明IDDM患者的肾上腺自身抗体是抗P450c21自身抗体。IDDM患者的P450c21自身抗体水平显著高于13例（9例P450c21自身抗体阳性）艾迪生病患者（P < 0.05）。虽然IDDM中P450c21抗体的发生率相对较低，但抗体阳性患者的抗体水平较高，可能存在肾上腺自身免疫的亚临床进程。
