表皮生长因子刺激的蛋白激酶p56 KKIAMRE的分子克隆

Molecular cloning of the epidermal growth factor-stimulated protein kinase p56 KKIAMRE.

作者信息

Taglienti C A, Wysk M, Davis R J

机构信息

Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01605, USA.

出版信息

Oncogene. 1996 Dec 19;13(12):2563-74.

PMID:9000130

Abstract

A 56 kDa protein kinase was molecularly cloned from human fetal brain. This protein kinase (p56 KKIAMRE) shares homology with p42 KKIALRE (Meyerson et al., 1992) and is related to the proline-directed protein kinase group of signal transducing enzymes. The p56 KKIAMRE and p42 KKIALRE protein kinases exhibit mutually exclusive expression in reproductive tissues; p56 KKIAMRE in testis and p42 KKIALRE in ovary. p56 KKIAMRE and p42 KKIALRE may therefore contribute to signal transduction within these highly differentiated tissues. p56 KKIAMRE and p42 KKIALRE are activated by treatment of cells with epidermal growth factor (EGF). Although p56 KKIAMRE and p42 KKIALRE contain the MAP kinase dual phosphorylation motif Thr-Xaa-Tyr (Thr-Asp-Tyr), phosphorylation on Thr and Tyr within this motif is not required for EGF-stimulated protein kinase activity.

摘要

从人胎儿大脑中分子克隆出一种56 kDa的蛋白激酶。这种蛋白激酶（p56 KKIAMRE）与p42 KKIALRE具有同源性（迈耶森等人，1992年），并且与信号转导酶的脯氨酸定向蛋白激酶组相关。p56 KKIAMRE和p42 KKIALRE蛋白激酶在生殖组织中呈现互斥性表达；p56 KKIAMRE在睾丸中表达，p42 KKIALRE在卵巢中表达。因此，p56 KKIAMRE和p42 KKIALRE可能在这些高度分化的组织中参与信号转导。用表皮生长因子（EGF）处理细胞可激活p56 KKIAMRE和p42 KKIALRE。尽管p56 KKIAMRE和p42 KKIALRE含有丝裂原活化蛋白激酶双磷酸化基序苏氨酸-任一氨基酸-酪氨酸（苏氨酸-天冬氨酸-酪氨酸），但该基序内苏氨酸和酪氨酸的磷酸化对于EGF刺激的蛋白激酶活性并非必需。