Rück A, Beck G, Bachor R, Akgün N, Gschwend M H, Steiner R
Institut für Lasertechnologien in der Medizin und Messtechnik, Ulm, Germany.
J Photochem Photobiol B. 1996 Nov;36(2):127-33. doi: 10.1016/s1011-1344(96)07359-9.
The fluorescence emission of hydrophilic tetrasulphonated aluminium phthalocyanine (AlPcS4) and hydrophobic zinc phthalocyanine (ZnPc), bound to the membrane of liposomes, was investigated in vivo in an appropriate tumour model of the rat bladder and in RR 1022 epithelial cells of the rat. The sensitizers were administered systemically to the rats and photodynamic therapy (PDT) was performed 24 h later. During PDT treatment, the fluorescence was measured every 30 s. The fluorescence was excited with 633 nm light from an HeNe laser and the fluorescence spectra were detected with an optical multichannel analyser system. PDT was performed for both sensitizers using 672 nm light from an Ar+ dye laser. The fluorescence changes during PDT were significantly different for the two phthalocyanines. For AlPcS4, an initial fluorescence intensity increase, followed by subsequent photobleaching, was observed. In contrast, ZnPc fluorescence showed an exponential decrease and no increase at the start of treatment. Tumour necrosis 24 h after PDT was significant only for ZnPc. RR 1022 cells incubated for 24 h with AlPcS4 revealed a granular fluorescence pattern, whereas ZnPc was localized diffusely in the cytoplasm of the cells. In agreement with the in vivo measurements, subcellular relocalization and a fluorescence intensity increase were detected exclusively in the case of AlPcS4. Morphological changes at this time were significant only for ZnPc. The subcellular localization and fluorescence kinetics were obtained using a confocal laser scanning microscope.
在大鼠膀胱的合适肿瘤模型以及大鼠的RR 1022上皮细胞中，对结合于脂质体膜上的亲水性四磺酸铝酞菁（AlPcS4）和疏水性锌酞菁（ZnPc）的荧光发射进行了体内研究。将敏化剂全身给予大鼠，并在24小时后进行光动力疗法（PDT）。在PDT治疗期间，每30秒测量一次荧光。用氦氖激光器发出的633nm光激发荧光，并用光学多通道分析仪系统检测荧光光谱。使用氩离子染料激光器发出的672nm光对两种敏化剂进行PDT。两种酞菁在PDT期间的荧光变化显著不同。对于AlPcS4，观察到初始荧光强度增加，随后是光漂白。相比之下，ZnPc荧光呈指数下降，在治疗开始时没有增加。PDT后24小时肿瘤坏死仅对ZnPc显著。用AlPcS4孵育24小时的RR 1022细胞显示出颗粒状荧光模式，而ZnPc则弥漫性地定位于细胞的细胞质中。与体内测量结果一致，仅在AlPcS4的情况下检测到亚细胞重新定位和荧光强度增加。此时的形态学变化仅对ZnPc显著。使用共聚焦激光扫描显微镜获得亚细胞定位和荧光动力学。
