van der Vlugt-Bergmans C J, Wagemakers C A, van Kan J A
Department of Phytopathology, Wageningen Agricultural University, The Netherlands.
Mol Plant Microbe Interact. 1997 Jan;10(1):21-9. doi: 10.1094/MPMI.1997.10.1.21.
Cutinase of Botrytis cinerea has been suggested to play an important role in penetration of host tissues. A protein fraction with cutin hydrolyzing activity was purified from culture filtrates of B. cinerea induced for cutinase activity. An 18-kDa protein in this fraction was identified as cutinase and the corresponding gene cutA was cloned. The gene is present in a single copy in the genome of B. cinerea strain SAS56 and its predicted amino acid sequence shows significant homology (31 to 35% identity) to other fungal cutinases. RNA blot analysis showed that cutA mRNA is induced in vitro by the cutin monomer 16-hydroxyhexadecanoic acid and repressed by glucose. The expression of cutA during infection of tomato leaves is low during early phases of infection, but high when the fungus has colonized the leaf and starts to sporulate.
灰葡萄孢的角质酶被认为在宿主组织的穿透过程中起重要作用。从诱导产生角质酶活性的灰葡萄孢培养滤液中纯化出一种具有角质水解活性的蛋白质组分。该组分中的一种18 kDa蛋白质被鉴定为角质酶，并克隆了相应的基因cutA。该基因在灰葡萄孢菌株SAS56的基因组中以单拷贝形式存在，其预测的氨基酸序列与其他真菌角质酶具有显著的同源性（同一性为31%至35%）。RNA印迹分析表明，cutA mRNA在体外被角质单体16-羟基十六烷酸诱导，并被葡萄糖抑制。在番茄叶片感染过程中，cutA在感染早期表达较低，但当真菌在叶片上定殖并开始产孢时表达较高。
