Skutelsky E, Bayer E A
Isr J Med Sci. 1979 Aug;15(8):675-86.
A novel approach for the ultrastructural localization of surface sialic acids is presented. Membrane-bound sialyl residues are chemically modified in situ by the covalent attachment of biotinyl residues, the latter of which are subsequently localized by ferritin-conjugated avidin. In contrast to previous methods, which have been based on electrostatic interactions, the above method does not affect cell surface charge. Consequently, the macromolecular configuration of the labeled sialoglycoconjugates is preserved. The method has been found to be more accurate in the quantitative evaluation and the topographical localization of membrane-based sialic acids both in normal and pathologically induced surface modulations. Modulations in cell surface sialic acid content and/or distribution have been demonstrated in beta-thalassemia and transformed lymphoid cells, and the consequences of such alterations are discussed regarding destruction vs. escape from the immune surveillance system.
本文提出了一种用于表面唾液酸超微结构定位的新方法。膜结合唾液酸残基通过生物素残基的共价连接在原位进行化学修饰，随后通过铁蛋白偶联抗生物素蛋白对后者进行定位。与以往基于静电相互作用的方法不同，上述方法不会影响细胞表面电荷。因此，标记的唾液酸糖缀合物的大分子构型得以保留。已发现该方法在正常和病理诱导的表面调节中对基于膜的唾液酸进行定量评估和拓扑定位时更为准确。在β地中海贫血和转化的淋巴细胞中已证实细胞表面唾液酸含量和/或分布的调节，并讨论了这种改变在破坏与逃避免疫监视系统方面的后果。
